Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody | Alomone Labs ASC-008-FR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody

catalog :

ASC-008-FR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Albrecht P, Houk G, Ruggiero E, Dockum M, Czerwinski M, Betts J, et al. Keratinocyte Biomarkers Distinguish Painful Diabetic Peripheral Neuropathy Patients and Correlate With Topical Lidocaine Responsiveness. Front Pain Res (Lausanne). 2021;2:790524 pubmed publisher
Voskobiynyk Y, Battu G, Felker S, Cochran J, Newton M, Lambert L, et al. Aberrant regulation of a poison exon caused by a non-coding variant in a mouse model of Scn1a-associated epileptic encephalopathy. PLoS Genet. 2021;17:e1009195 pubmed publisher

image

image 1 :

Expression of NaV1.7 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR). A. NaV1.7 staining (purple) appears in DRG neurons. B. Nuclear staining using DAPI as the counterstain. C. Merge images of A and B.

product information

CAT :

ASC-008-FR

SKU :

ASC-008-FR_50 mcl

Product Name :

Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08562

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC008

Homology :

Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular loop between domains I and II

Label :

ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Short Description :

A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel Conjugated to the Fluorescent Dye ATTO-633

Long Description :

Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.7 from rat, human, and mouse samples. Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-FR

Positive Control :

Synonyms :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN9A

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASC-008), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage