Anti-NaV1.5 (SCN5A) (493-511) Antibody | Alomone Labs ASC-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.5 (SCN5A) (493-511) Antibody

catalog :

ASC-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 45

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 4a immunocytochemistry; rat; loading ...; fig 4c western blot; rat; 1:200; loading ...; fig 4a	Liu X, Ge J, Chen C, Shen Y, Xie J, Zhu X, et al. FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation. Cell Death Dis. 2021;12:25 pubmed publisher
immunohistochemistry - frozen section; rat; 1:50; loading ...; fig 5a	Xiao Y, Cai X, Atkinson A, Logantha S, Boyett M, Dobrzynski H. Expression of connexin 43, ion channels and Ca2+-handling proteins in rat pulmonary vein cardiomyocytes. Exp Ther Med. 2016;12:3233-3241 pubmed
immunohistochemistry; mouse; 1:50; loading ...; fig 1b western blot; mouse; 1:500; loading ...; fig 7d	Shekhar A, Lin X, Liu F, Zhang J, Mo H, Bastarache L, et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest. 2016;126:4444-4459 pubmed publisher
	Kabakov A, Sengun E, Lu Y, Roder K, Bronk P, Baggett B, et al. Three-Week-Old Rabbit Ventricular Cardiomyocytes as a Novel System to Study Cardiac Excitation and EC Coupling. Front Physiol. 2021;12:672360 pubmed publisher
	Li J, Li Y, Liu Y, Yu H, Xu N, Huang D, et al. Fibroblast Growth Factor 21 Ameliorates NaV1.5 and Kir2.1 Channel Dysregulation in Human AC16 Cardiomyocytes. Front Pharmacol. 2021;12:715466 pubmed publisher
	Zhang D, Navara R, Yin T, SZYMANSKI J, Goldsztejn U, Kenkel C, et al. Cardiac radiotherapy induces electrical conduction reprogramming in the absence of transmural fibrosis. Nat Commun. 2021;12:5558 pubmed publisher
	Pierre M, Djemai M, Poulin H, Chahine M. NaV1.5 knockout in iPSCs: a novel approach to study NaV1.5 variants in a human cardiomyocyte environment. Sci Rep. 2021;11:17168 pubmed publisher
	Liu M, Liu H, Parthiban P, Kang G, Shi G, Feng F, et al. Inhibition of the unfolded protein response reduces arrhythmia risk after myocardial infarction. J Clin Invest. 2021;131: pubmed publisher
	Turan N, Moshal K, Roder K, Baggett B, Kabakov A, Dhakal S, et al. The endosomal trafficking regulator LITAF controls the cardiac Nav1.5 channel via the ubiquitin ligase NEDD4-2. J Biol Chem. 2020;295:18148-18159 pubmed publisher
	Abrams J, Roybal D, Chakouri N, Katchman A, Weinberg R, Yang L, et al. Fibroblast growth factor homologous factors tune arrhythmogenic late NaV1.5 current in calmodulin binding-deficient channels. JCI Insight. 2020;5: pubmed publisher
	Khoo K, Galleano I, Gasparri F, Wieneke R, Harms H, Poulsen M, et al. Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. Nat Commun. 2020;11:2284 pubmed publisher
	Matasic D, Yoon J, McLendon J, Mehdi H, Schmidt M, Greiner A, et al. Modulation of the cardiac sodium channel NaV1.5 peak and late currents by NAD+ precursors. J Mol Cell Cardiol. 2020;141:70-81 pubmed publisher
	Shimizu A, Zankov D, Sato A, Komeno M, Toyoda F, Yamazaki S, et al. Identification of transmembrane protein 168 mutation in familial Brugada syndrome. FASEB J. 2020;34:6399-6417 pubmed publisher
	Martin B, Gabris B, Barakat A, Henry B, Giannini M, Reddy R, et al. Relaxin reverses maladaptive remodeling of the aged heart through Wnt-signaling. Sci Rep. 2019;9:18545 pubmed publisher
	Eichel C, R os P rez E, Liu F, Jameson M, Jones D, Knickelbine J, et al. A microtranslatome coordinately regulates sodium and potassium currents in the human heart. elife. 2019;8: pubmed publisher
	Casini S, Albesa M, Wang Z, Portero V, Ross Kaschitza D, Rougier J, et al. Functional Consequences of the SCN5A-p.Y1977N Mutation within the PY Ubiquitylation Motif: Discrepancy between HEK293 Cells and Transgenic Mice. Int J Mol Sci. 2019;20: pubmed publisher
	Cortada E, Brugada R, Verges M. N-Glycosylation of the voltage-gated sodium channel β2 subunit is required for efficient trafficking of NaV1.5/β2 to the plasma membrane. J Biol Chem. 2019;294:16123-16140 pubmed publisher
	Zhao L, Sun L, Lu Y, Li F, Xu H. A small-molecule LF3 abrogates β-catenin/TCF4-mediated suppression of NaV1.5 expression in HL-1 cardiomyocytes. J Mol Cell Cardiol. 2019;135:90-96 pubmed publisher
	Sengupta S, Rothenberg K, Li H, Hoffman B, Bursac N. Altering integrin engagement regulates membrane localization of Kir2.1 channels. J Cell Sci. 2019;132: pubmed publisher
	de la Roche J, Angsutararux P, Kempf H, Janan M, Bolesani E, Thiemann S, et al. Comparing human iPSC-cardiomyocytes versus HEK293T cells unveils disease-causing effects of Brugada mutation A735V of NaV1.5 sodium channels. Sci Rep. 2019;9:11173 pubmed publisher
	Salvarani N, Crasto S, Miragoli M, Bertero A, Paulis M, Kunderfranco P, et al. The K219T-Lamin mutation induces conduction defects through epigenetic inhibition of SCN5A in human cardiac laminopathy. Nat Commun. 2019;10:2267 pubmed publisher
	Menon A, Hong L, Savio Galimberti E, Sridhar A, Youn S, Zhang M, et al. Electrophysiologic and molecular mechanisms of a frameshift NPPA mutation linked with familial atrial fibrillation. J Mol Cell Cardiol. 2019;132:24-35 pubmed publisher
	Mlynarova J, Trentin Sonoda M, Gaisler da Silva F, Major J, Salih M, Carneiro Ramos M, et al. SLMAP3 isoform modulates cardiac gene expression and function. PLoS ONE. 2019;14:e0214669 pubmed publisher
	Tang B, Hu Y, Wang Z, Cheng C, Wang P, Liang L, et al. UBC9 regulates cardiac sodium channel Nav1.5 ubiquitination, degradation and sodium current density. J Mol Cell Cardiol. 2019;129:79-91 pubmed publisher
	Han M, Zhao M, Cheng C, Huang Y, Han S, Li W, et al. Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation. Hum Mutat. 2019;40:310-325 pubmed publisher
	Ahmad S, Tirilomis P, Pabel S, Dybkova N, Hartmann N, Molina C, et al. The functional consequences of sodium channel NaV 1.8 in human left ventricular hypertrophy. ESC Heart Fail. 2019;6:154-163 pubmed publisher
	Motloch L, Cacheux M, Ishikawa K, Xie C, Hu J, Aguero J, et al. Primary Effect of SERCA 2a Gene Transfer on Conduction Reserve in Chronic Myocardial Infarction. J Am Heart Assoc. 2018;7:e009598 pubmed publisher
	Yu G, Liu Y, Qin J, Wang Z, Hu Y, Wang F, et al. Mechanistic insights into the interaction of the MOG1 protein with the cardiac sodium channel Nav1.5 clarify the molecular basis of Brugada syndrome. J Biol Chem. 2018;293:18207-18217 pubmed publisher
	Wang Z, Yu G, Liu Y, Liu S, Aridor M, Huang Y, et al. Small GTPases SAR1A and SAR1B regulate the trafficking of the cardiac sodium channel Nav1.5. Biochim Biophys Acta Mol Basis Dis. 2018;1864:3672-3684 pubmed publisher
	Chen X, Zhu C, Zhou H, Zhang Y, Cai Z, Wu H, et al. Key Role of the Membrane Trafficking of Nav1.5 Channel Protein in Antidepressant-Induced Brugada Syndrome. Front Physiol. 2018;9:1230 pubmed publisher
	Pappalardo L, Samad O, Liu S, Zwinger P, Black J, Waxman S. Nav1.5 in astrocytes plays a sex-specific role in clinical outcomes in a mouse model of multiple sclerosis. Glia. 2018;66:2174-2187 pubmed publisher
	Li G, Khandekar A, Yin T, Hicks S, Guo Q, Takahashi K, et al. Differential Wnt-mediated programming and arrhythmogenesis in right versus left ventricles. J Mol Cell Cardiol. 2018;123:92-107 pubmed publisher
	Shekhar A, Lin X, Lin B, Liu F, Zhang J, Khodadadi Jamayran A, et al. ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes. Sci Rep. 2018;8:9944 pubmed publisher
	Malmo V, Kelly A, Garten K, Stolen T, Rolim N, Wisloff U, et al. Aerobic Interval Training Prevents Age-Dependent Vulnerability to Atrial Fibrillation in Rodents. Front Physiol. 2018;9:206 pubmed publisher
	Zhang X, Yoon J, Morley M, McLendon J, Mapuskar K, Gutmann R, et al. A common variant alters SCN5A-miR-24 interaction and associates with heart failure mortality. J Clin Invest. 2018;128:1154-1163 pubmed publisher
	Xia P, Li L, Qi R, Shi J, Ju W, Chen M. Electrical and histological remodeling of the pulmonary vein in 2K1C hypertensive rats: Indication of initiation and maintenance of atrial fibrillation. Anatol J Cardiol. 2018;19:169-175 pubmed publisher
	Huang L, Wu K, Zhang L, Wang Q, Tang S, Wu Q, et al. Critical Roles of Xirp Proteins in Cardiac Conduction and Their Rare Variants Identified in Sudden Unexplained Nocturnal Death Syndrome and Brugada Syndrome in Chinese Han Population. J Am Heart Assoc. 2018;7: pubmed publisher
	Burel S, Coyan F, Lorenzini M, Meyer M, Lichti C, BROWN J, et al. C-terminal phosphorylation of NaV1.5 impairs FGF13-dependent regulation of channel inactivation. J Biol Chem. 2017;292:17431-17448 pubmed publisher
	Papp R, Bett G, Lis A, Rasmusson R, Baczkó I, Varro A, et al. Genomic upregulation of cardiac Cav1.2? and NCX1 by estrogen in women. Biol Sex Differ. 2017;8:26 pubmed publisher
	Qiao Y, Lipovsky C, HICKS S, Bhatnagar S, Li G, Khandekar A, et al. Transient Notch Activation Induces Long-Term Gene Expression Changes Leading to Sick Sinus Syndrome in Mice. Circ Res. 2017;121:549-563 pubmed publisher
	Vikram A, Lewarchik C, Yoon J, Naqvi A, Kumar S, Morgan G, et al. Sirtuin 1 regulates cardiac electrical activity by deacetylating the cardiac sodium channel. Nat Med. 2017;23:361-367 pubmed publisher
	Xu Q, Patel D, Zhang X, Veenstra R. Changes in cardiac Nav1.5 expression, function, and acetylation by pan-histone deacetylase inhibitors. Am J Physiol Heart Circ Physiol. 2016;311:H1139-H1149 pubmed publisher
	Wang N, Huo R, Cai B, Lu Y, Ye B, Li X, et al. Activation of Wnt/?-catenin signaling by hydrogen peroxide transcriptionally inhibits NaV1.5 expression. Free Radic Biol Med. 2016;96:34-44 pubmed publisher
	Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher
	Malan D, Zhang M, Stallmeyer B, MÃ¼ller J, Fleischmann B, Schulze Bahr E, et al. Human iPS cell model of type 3 long QT syndrome recapitulates drug-based phenotype correction. Basic Res Cardiol. 2016;111:14 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200). 2. Anti-NaV1.5 (SCN5A) (493-511) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).

image 2 :

Expression of NaV1.5 in rat heart - Immunohistochemical staining of NaV1.5 in rat myocardium paraffin-embedded sections using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the small vessels are negative (red arrows). Hematoxilin is used as the counterstain.

image 3 :

Peptide DRLPKSDSEDGPRALNQLS(C) corresponding to amino acid residues 493-511 ofrat NaV1.5 (accession numberP15389). Intracellular loop between domains I and II.

product information

CAT :

ASC-005

SKU :

ASC-005-CF_0.2 ml

Product Name :

Anti-NaV1.5 (SCN5A) (493-511) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15389

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC005

Homology :

Mouse - identical; human - 17/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains I and II

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 of rat NaV1.5

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.1 Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.5.2The putative structure of NaV1.5 consists of four homologous domains (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome.1-2 Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system.1

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Sodium channel protein type 5 subunit alpha, SKM2

Short Description :

A Rabbit Polyclonal Antibody to NaV1.5 (SCN5A) (493-511) Channel

Long Description :

Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human, and mouse samples.

Negative Control :

BLP-SC005

Positive Control :

Synonyms :

Sodium channel protein type 5 subunit alpha, SKM2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN5A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage