Guinea pig Anti-NaV1.5 (SCN5A) Antibody | Alomone Labs ASC-005-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-NaV1.5 (SCN5A) Antibody

catalog :

ASC-005-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 10

Reference
Gao R, Cao T, Chen H, Cai J, Lei M, Wang Z. Nav1.5-E3 antibody inhibits cancer progression. Transl Cancer Res. 2019;8:44-50 pubmed publisher
Zhang D, Navara R, Yin T, SZYMANSKI J, Goldsztejn U, Kenkel C, et al. Cardiac radiotherapy induces electrical conduction reprogramming in the absence of transmural fibrosis. Nat Commun. 2021;12:5558 pubmed publisher
Martin B, Gabris B, Barakat A, Henry B, Giannini M, Reddy R, et al. Relaxin reverses maladaptive remodeling of the aged heart through Wnt-signaling. Sci Rep. 2019;9:18545 pubmed publisher
Cacheux M, Strauss B, Raad N, Ilkan Z, Hu J, Benard L, et al. Cardiomyocyte-Specific STIM1 (Stromal Interaction Molecule 1) Depletion in the Adult Heart Promotes the Development of Arrhythmogenic Discordant Alternans. Circ Arrhythm Electrophysiol. 2019;12:e007382 pubmed publisher
Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
Yang Q, Xiong H, Xu C, Huang Y, Tu X, Wu G, et al. Identification of rare variants in cardiac sodium channel β4-subunit gene SCN4B associated with ventricular tachycardia. Mol Genet Genomics. 2019;: pubmed publisher
Guan Y, Gao X, Tang Q, Huang L, Gao S, Yu S, et al. Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity. J Cell Mol Med. 2019;23:1448-1457 pubmed publisher
Lai Y, Li N, Lawrence W, Wang S, Levine A, Burchhardt D, et al. Myocardial remodeling and susceptibility to ventricular tachycardia in a model of chronic epilepsy. Epilepsia Open. 2018;3:213-223 pubmed publisher
Cheng J, Kyle J, Lang D, Wiedmeyer B, Guo J, Yin K, et al. An East Asian Common Variant Vinculin P.Asp841His Was Associated With Sudden Unexplained Nocturnal Death Syndrome in the Chinese Han Population. J Am Heart Assoc. 2017;6: pubmed publisher
Cheng J, Kyle J, Wiedmeyer B, Lang D, Vaidyanathan R, Makielski J. Vinculin variant M94I identified in sudden unexplained nocturnal death syndrome decreases cardiac sodium current. Sci Rep. 2017;7:42953 pubmed publisher

image

image 1 :

Western blot analysis of human-NaV1.5 transfected HEK-293 cell lysate (lanes 1 and 3) and rat heart membrane protein (lanes 2 and 4): - 1,2. Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#ASC-005-GP), (1:200).3,4. Guinea pig Anti-NaV1.5 (SCN5A) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).

image 2 :

Expression of NaV1.5 in rat heart - Immunohistochemical staining of rat heart frozen sections using Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#ASC-005-GP). NaV1.5 staining (red) appears in cardiomyocytes and in intercalated discs (arrows). Nuclei are stained using DAPI (blue).

product information

CAT :

ASC-005-GP

SKU :

ASC-005-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-NaV1.5 (SCN5A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15389

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-SC005

Homology :

Mouse - identical; human - 17/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains I and II

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 of rat NaV1.5

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability1.Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.52.The putative structure of NaV1.5 consists of four homologous domain (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome1,2. Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Sodium channel protein type 5 subunit alpha, SKM2

Short Description :

A Guinea Pig Polyclonal Antibody to NaV1.5 (SCN5A) (493-511) Channel

Long Description :

Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#ASC-005-GP, formerly AGP-008), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-SC005

Positive Control :

Synonyms :

Sodium channel protein type 5 subunit alpha, SKM2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN5A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage