Anti-SCN3A (NaV1.3) Antibody | Alomone Labs ASC-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN3A (NaV1.3) Antibody

catalog :

ASC-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 12

Reference
Mulpuri Y, Yamamoto T, Nishimura I, Spigelman I. Role of voltage-gated sodium channels in axonal signal propagation of trigeminal ganglion neurons after infraorbital nerve entrapment. Neurobiol Pain. 2022;11:100084 pubmed publisher
R xe9 thor xe9 L, Park J, Montnach J, Nicolas S, Khoury J, Le Seac h E, et al. Pharmacological Dissection of the Crosstalk between NaV and CaV Channels in GH3b6 Cells. Int J Mol Sci. 2022;23: pubmed publisher
Helm M, Dankovich T, Mandad S, Rammner B, Jähne S, Salimi V, et al. A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines. Nat Neurosci. 2021;24:1151-1162 pubmed publisher
Zhang Q, Martin Caraballo M, Hsia S. Modulation of Voltage-Gated Sodium Channel Activity in Human Dorsal Root Ganglion Neurons by Herpesvirus Quiescent Infection. J Virol. 2020;94: pubmed publisher
Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
Koleske M, Bonilla I, Thomas J, Zaman N, Baine S, Knollmann B, et al. Tetrodotoxin-sensitive Navs contribute to early and delayed afterdepolarizations in long QT arrhythmia models. J Gen Physiol. 2018;150:991-1002 pubmed publisher
Guan G, Zhao M, Xu X, Boczek T, Mao X, Li Z, et al. Abnormal changes in voltage-gated sodium channels subtypes NaV1.1, NaV1.2, NaV1.3, NaV1.6 and CaM/CaMKII pathway in low-grade astrocytoma. Neurosci Lett. 2018;674:148-155 pubmed publisher
Chen K, Yang C, Wallace C, Lin C, Liu C, Yin T, et al. Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat. Am J Transl Res. 2017;9:4593-4606 pubmed
Lamar T, Vanoye C, Calhoun J, Wong J, Dutton S, Jorge B, et al. SCN3A deficiency associated with increased seizure susceptibility. Neurobiol Dis. 2017;102:38-48 pubmed publisher
Yang K, Kim M, Ju J, Park S, Lee C, Kim S, et al. Antinociceptive Effects of Botulinum Toxin Type A on Trigeminal Neuropathic Pain. J Dent Res. 2016;95:1183-90 pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat newborn brain membranes: - 1. Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:200).2. Anti-SCN3A (NaV1.3) Antibody, preincubated with SCN3A/Nav1.3 Blocking Peptide (#BLP-SC004).

image 2 :

Expression of NaV1.3 in rat embryo DRG - Immunohistochemical staining of rat embryo dorsal root ganglion (DRG) frozen sections using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:100). NaV1.3 is expressed in DRG embryonic cells (arrows). Calibration bar = 50 µm.

image 3 :

Expression of NaV1.3 in rat embryo DRG - Immunohistochemical staining of rat embryo dorsal root ganglion (DRG) frozen sections usingAnti-SCN3A (NaV1.3)Antibody (#ASC-004) (1:100). NaV1.3 is expressed in DRG embryonic cells (arrows). Calibration bar = 50 m.

product information

CAT :

ASC-004

SKU :

ASC-004-CF_0.2 ml

Product Name :

Anti-SCN3A (NaV1.3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08104

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC004

Homology :

Mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, loop between domains I and II

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat NaV1.3

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Short Description :

A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel

Long Description :

Anti-SCN3A (NaV1.3) Antibody (#ASC-004) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.3 from rat, human, and mouse samples.

Negative Control :

BLP-SC004

Positive Control :

Synonyms :

BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN3A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage