Anti-Pan NaV Antibody | Alomone Labs ASC-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Pan NaV Antibody

catalog :

ASC-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 13

Reference
Kwan H, Chan Z, Bi X, Kutkowska J, Pr xf3 szy x144 ski T, Chan C, et al. Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3. Sci Adv. 2023;9:eadg0183 pubmed publisher
Teunissen M, Lewerissa E, van Hugte E, Wang S, Ockeloen C, Koolen D, et al. ANK2 loss-of-function variants are associated with epilepsy, and lead to impaired axon initial segment plasticity and hyperactive network activity in hiPSC-derived neuronal networks. Hum Mol Genet. 2023;32:2373-2385 pubmed publisher
Pierre M, Djemai M, Poulin H, Chahine M. NaV1.5 knockout in iPSCs: a novel approach to study NaV1.5 variants in a human cardiomyocyte environment. Sci Rep. 2021;11:17168 pubmed publisher
Voskobiynyk Y, Battu G, Felker S, Cochran J, Newton M, Lambert L, et al. Aberrant regulation of a poison exon caused by a non-coding variant in a mouse model of Scn1a-associated epileptic encephalopathy. PLoS Genet. 2021;17:e1009195 pubmed publisher
Paul D, Maggi P, Piero F, Scahill S, Sherman K, Edenfield S, et al. Targeted Osmotic Lysis of Highly Invasive Breast Carcinomas Using Pulsed Magnetic Field Stimulation of Voltage-Gated Sodium Channels and Pharmacological Blockade of Sodium Pumps. Cancers (Basel). 2020;12: pubmed publisher
Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
Motipally S, Allen K, Williamson D, Marsat G. Differences in Sodium Channel Densities in the Apical Dendrites of Pyramidal Cells of the Electrosensory Lateral Line Lobe. Front Neural Circuits. 2019;13:41 pubmed publisher
Bernal L, Cisneros E, García Magro N, Roza C. Immunostaining in whole-mount lipid-cleared peripheral nerves and dorsal root ganglia after neuropathy in mice. Sci Rep. 2019;9:8374 pubmed publisher
Muniswami D, Kanthakumar P, Kanakasabapathy I, Tharion G. Motor Recovery after Transplantation of Bone Marrow Mesenchymal Stem Cells in Rat Models of Spinal Cord Injury. Ann Neurosci. 2019;25:126-140 pubmed publisher
Hanada Y, Nakamura Y, Ozono Y, Ishida Y, Takimoto Y, Taniguchi M, et al. Fibroblast growth factor 12 is expressed in spiral and vestibular ganglia and necessary for auditory and equilibrium function. Sci Rep. 2018;8:11491 pubmed publisher
Johnson K, Herold K, Milner T, Hemmings H, Platholi J. Sodium channel subtypes are differentially localized to pre- and post-synaptic sites in rat hippocampus. J Comp Neurol. 2017;525:3563-3578 pubmed publisher
Dustrude E, Moutal A, Yang X, Wang Y, Khanna M, Khanna R. Hierarchical CRMP2 posttranslational modifications control NaV1.7 function. Proc Natl Acad Sci U S A. 2016;113:E8443-E8452 pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Pan NaV Antibody (#ASC-003), (1:200).2. Anti-Pan NaV Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).

image 2 :

Expression of NaV channels in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-Pan NaV Antibody (#ASC-003). A. NaV channels (red) appear in Purkinje cell bodies (vertical arrows) and in cells of the molecular layer (Mol), (horizontal arrows). B. Staining of Parvalbumin (green) in the same brain section. C. Confocal merge of NaV channels and Parvalbumin demonstrates the location of NaV channels to the cell body of Purkinje neurons.

image 3 :

Expression ofNaVchannels in rat cerebellum - Immunohistochemical staining of rat cerebellum usingAnti-Pan NaVAntibody (#ASC-003). A. NaVchannels (red) appear in Purkinje cell bodies (vertical arrows) and in cells of the molecular layer (Mol) (horizontal arrows). B. Staining of Parvalbumin (green) in the same brain section. C. Confocal merge of NaVchannels and Parvalbumin demonstrates the location of NaVchannels to the cell body of Purkinje neurons.

product information

CAT :

ASC-003

SKU :

ASC-003-CF_0.2 ml

Product Name :

Anti-Pan NaV Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P04774

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC003

Homology :

The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains III and IV

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Pan sodium channel, SP19

Short Description :

A Rabbit Polyclonal Antibody to Pan NaV Channel

Long Description :

Alomone Labs is pleased to offer an antibody directed against an intracellular epitope of the rat NaV1.1 channel that is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels. Anti-Pan NaV Antibody (#ASC-003) can be used in western blot, immunocytochemistry, and immunohistochemistry applications.

Negative Control :

BLP-SC003

Positive Control :

Synonyms :

Pan sodium channel, SP19

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN1A, SCN2A, SCN3A, SCN4A, SCN5A, SCN8A, SCN9A, SCN10A, SCN11A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC Whole Tissue Staining

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage