Guinea pig Anti-Pan NaV Antibody | Alomone Labs ASC-003-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Pan NaV Antibody

catalog :

ASC-003-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Gupta S, Bazargani N, Drew J, Howden J, Modi S, Al Awabdh S, et al. The non-adrenergic imidazoline-1 receptor protein nischarin is a key regulator of astrocyte glutamate uptake. iScience. 2022;25:104127 pubmed publisher
Voskobiynyk Y, Battu G, Felker S, Cochran J, Newton M, Lambert L, et al. Aberrant regulation of a poison exon caused by a non-coding variant in a mouse model of Scn1a-associated epileptic encephalopathy. PLoS Genet. 2021;17:e1009195 pubmed publisher
Madden J, Davis O, Boyle K, Iredale J, Browne T, Callister R, et al. Functional and Molecular Analysis of Proprioceptive Sensory Neuron Excitability in Mice. Front Mol Neurosci. 2020;13:36 pubmed publisher
Orefice L, Mosko J, Morency D, Wells M, Tasnim A, Mozeika S, et al. Targeting Peripheral Somatosensory Neurons to Improve Tactile-Related Phenotypes in ASD Models. Cell. 2019;178:867-886.e24 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 1,2. Guinea pig Anti-Pan NaV Antibody (#ASC-003-GP), (1:800).3,4. Guinea pig Anti-Pan NaV Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).

product information

CAT :

ASC-003-GP

SKU :

ASC-003-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Pan NaV Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P04774

Applications :

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-SC003

Homology :

The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains III and IV

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

Applications may also work in :

Supplier :

Alomone Labs

Target :

Pan sodium channel, SP19

Short Description :

A Guinea Pig Polyclonal Antibody to Pan NaV Channel

Long Description :

Alomone Labs is pleased to offer an antibody directed against an epitope of the rat NaV1.1 channel. The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels. Guinea pig Anti-Pan NaV Antibody (#ASC-003-GP, formerly AGP-007), raised in guinea pigs, can be used in western blot analysis, and has been designed to recognize NaV channels from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Pan NaV Antibody (#ASC-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-SC003

Positive Control :

Synonyms :

Pan sodium channel, SP19

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN1A, SCN2A, SCN3A, SCN4A, SCN5A, SCN8A, SCN9A, SCN10A, SCN11A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage