Anti-SCN2A (NaV1.2) Antibody | Alomone Labs ASC-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN2A (NaV1.2) Antibody

catalog :

ASC-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; loading ...; fig s3a	Lund C, Yellapragada V, Vuoristo S, Balboa D, Trova S, Allet C, et al. Characterization of the human GnRH neuron developmental transcriptome using a GNRH1-TdTomato reporter line in human pluripotent stem cells. Dis Model Mech. 2020;13: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:300; fig 8	Alshammari M, Alshammari T, Laezza F. Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment. Front Cell Neurosci. 2016;10:5 pubmed publisher
	Zhang J, Chen X, Eaton M, Wu J, Ma Z, Lai S, et al. Severe deficiency of the voltage-gated sodium channel NaV1.2 elevates neuronal excitability in adult mice. Cell Rep. 2021;36:109495 pubmed publisher
	Hikima T, Lee C, Witkovsky P, Chesler J, Ichtchenko K, Rice M. Activity-dependent somatodendritic dopamine release in the substantia nigra autoinhibits the releasing neuron. Cell Rep. 2021;35:108951 pubmed publisher
	Ciccone R, Franco C, Piccialli I, Boscia F, Casamassa A, de Rosa V, et al. Amyloid β-Induced Upregulation of Nav1.6 Underlies Neuronal Hyperactivity in Tg2576 Alzheimer's Disease Mouse Model. Sci Rep. 2019;9:13592 pubmed publisher
	Shin W, Kweon H, Kang R, Kim D, Kim K, Kang M, et al. Scn2a Haploinsufficiency in Mice Suppresses Hippocampal Neuronal Excitability, Excitatory Synaptic Drive, and Long-Term Potentiation, and Spatial Learning and Memory. Front Mol Neurosci. 2019;12:145 pubmed publisher
	Ogiwara I, Miyamoto H, Tatsukawa T, Yamagata T, Nakayama T, Atapour N, et al. Nav1.2 haplodeficiency in excitatory neurons causes absence-like seizures in mice. Commun Biol. 2018;1: pubmed publisher
	Katz E, Stoler O, Scheller A, Khrapunsky Y, Goebbels S, Kirchhoff F, et al. Role of sodium channel subtype in action potential generation by neocortical pyramidal neurons. Proc Natl Acad Sci U S A. 2018;115:E7184-E7192 pubmed publisher
	Guan G, Zhao M, Xu X, Boczek T, Mao X, Li Z, et al. Abnormal changes in voltage-gated sodium channels subtypes NaV1.1, NaV1.2, NaV1.3, NaV1.6 and CaM/CaMKII pathway in low-grade astrocytoma. Neurosci Lett. 2018;674:148-155 pubmed publisher
	Yamagata T, Ogiwara I, Mazaki E, Yanagawa Y, Yamakawa K. Nav1.2 is expressed in caudal ganglionic eminence-derived disinhibitory interneurons: Mutually exclusive distributions of Nav1.1 and Nav1.2. Biochem Biophys Res Commun. 2017;491:1070-1076 pubmed publisher
	Johnson K, Herold K, Milner T, Hemmings H, Platholi J. Sodium channel subtypes are differentially localized to pre- and post-synaptic sites in rat hippocampus. J Comp Neurol. 2017;525:3563-3578 pubmed publisher
	Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher
	Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-SCN2A (NaV1.2) Antibody (#ASC-002), (1:200).2. Anti-SCN2A (NaV1.2) Antibody, preincubated with SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002).

image 2 :

Expression of NaV1.2 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus using Anti-SCN2A (NaV1.2) Antibody (#ASC-002 ). A. NaV1.2 (red) is present in dendrites of pyramidal neurons in the CA3 region. B. Staining of interneurons in the pyramidal layer with mouse anti-Parvalbumin (green) demonstrates the restriction of NaV1.2 to dendrites (arrows) extending from the pyramidal layer (P). C. Confocal merge of panels A and B.

image 3 :

Immunoprecipitation of rat brain lysate: - 1. Rat brain lysates2. Lysates immunoprecipitated withAnti-SCN2A (NaV1.2)Antibody (#ASC-002) (6 g).3. Lysates immunoprecipitated with pre-immune rabbit serum.The lower arrow indicates the IgG heavy chain.Western blot analysiswas performed with Anti-SCN2A (NaV1.2) Antibody.

product information

CAT :

ASC-002

SKU :

ASC-002-CF_0.2 ml

Product Name :

Anti-SCN2A (NaV1.2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P04775

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC002

Homology :

Mouse, Human - identicalNaV13 - 12 out of 19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains I and II

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7NaV1.2, also known as SCN2A, is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the NaV1.2 channel have been identified in different types of epilepsy.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha

Short Description :

A Rabbit Polyclonal Antibody to SCN2A (NaV1.2) Channel

Long Description :

Anti-SCN2A (NaV1.2) Antibody (#ASC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.2 from rat, human, and mouse samples.

Negative Control :

BLP-SC002

Positive Control :

Synonyms :

BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN2A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage