Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody | Alomone Labs ASC-001-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody

catalog :

ASC-001-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of NaV1.1 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody (#ASC-001-AR). A. Staining of NaV1.1 (red) is mainly in the molecular layer (Mol). B. DAPI is used as the counterstain (blue). C. Merge images of A and B.

image 2 :

Expression of NaV1.1 in rat cerebellum - Immunohistochemical staining ofrat cerebellum usingAnti-SCN1A (NaV1.1)-ATTO-594Antibody (#ASC-001-AR). A. Staining of NaV1.1 (red) is mainly inthe molecular layer (Mol). B. DAPI is used as the counterstain (blue). C. Merge images of A and B.

product information

CAT :

ASC-001-AR

SKU :

ASC-001-AR_50 mcl

Product Name :

Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P04774

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC001

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular loop between domains I and II

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,7.NaV1.1, also referred to as SCN1A, is a tetrodotoxin-sensitive channel and is broadly expressed in neurons7.Mutations in NaV1.1 are associated with at least two forms of epilepsy. Gain-of-function missense mutations are a primary cause of generalized epilepsy with febrile seizures plus (GEFS+). Loss-of-function mutations cause severe myoclonic epilepsy of infancy (SMEI)8,9.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α

Short Description :

A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-SCN1A (NaV1.1) Antibody (#ASC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.1 from rat, human, and mouse samples. Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody (#ASC-001-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-SC001

Positive Control :

Synonyms :

Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN1A

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASC-001), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage