Anti-RAMP1 (extracellular) Antibody | Alomone Labs ARR-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-RAMP1 (extracellular) Antibody

catalog :

ARR-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat pancreas (lanes 3 and 6): - 1-3. Anti-RAMP1 (extracellular) Antibody (#ARR-021).4-6. Anti-RAMP1 (extracellular) Antibody, preincubated with RAMP1 (extracellular) Blocking Peptide (#BLP-RR021). The antibody was used at a concentration of 1:400 for brain lysates and 1:200 for pancreas lysates.

image 2 :

Expression of RAMP1 in rat brain - Immunohistochemical staining of rat free-floating frozen section using Anti-RAMP1 (extracellular) Antibody (#ARR-021). A. In the parietal cortex, RAMP1 immunoreactivity (green) appears in pyramidal neurons (arrows). B. In the hippocampus, RAMP1 staining (green) is detected in the CA1 region (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).

product information

CAT :

ARR-021

SKU :

ARR-021-CF_0.2 ml

Product Name :

Anti-RAMP1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JJ74

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-RR021

Homology :

Mouse - identical; human - not recommended for human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRDPDYGTLIQE, corresponding to amino acid residues 27-38 of rat RAMP1

Product Page - Scientific background :

Receptor activity modifying protein-1 (RAMP1) is part of a three-member group (that further includes RAMP2 and RAMP3 - each encoded by a different gene) of transmembrane proteins that interact with G-protein coupled receptors (GPCRs) such as the calcitonin receptor-like receptors (CLRs)1. This interaction leads to the formation of the receptor for calcitonin gene related peptide (CGRP) and is necessary for the binding of the peptide to the receptor. The binding of RAMP2 and RAMP3 to CLR, on the other hand, creates the receptor for adrenomedullin, a peptide functionally and structurally related to calcitonin1,2.RAMP1 (as well as the two other RAMPs) has a short intracellular domain and a single transmembrane domain. Its extracellular region contains four conserved cysteine residues that putatively form two disulfide bonds. This domain confers specificity to the receptor complex, whereas the transmembrane chain allows the association of the RAMPs with the GPCRs. In contrast to the other RAMPs, RAMP1 lacks glycosylation sites and therefore is unable to migrate to the cell surface independently. To reach the cell surface, it aggregates inside the cytoplasm and forms heterodimers with calcitonin3.RAMP1 is highly prominent in the neuronal tissues (especially the trigeminal ganglion, striatum, cortex, olfactory bulb, dorsal root ganglion, and the spinal cord) but abundant as well in the electrical conducting system of the heart, blood vessels, and related sites1,2,4.While not dramatically affected, RAMP1-KO mice are reported to have higher blood pressure and to suffer from repressed wound healing process, while overexpression of RAMP1 in animals leads to extravasation and vasodilation2. Interestingly, asthmatic symptoms in mice model of asthma were mitigated in mice that were RAMP1-deficient4. Furthermore mesenchymal stem cells overexpressing RAMP1 were shown to improve cardiac function and to reduce occurrences of over-thickened blood vessels5.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Receptor activity-modifying protein 1, Calcitonin receptor-like receptor activity-modifying protein 1

Short Description :

A Rabbit Polyclonal Antibody to RAMP1

Long Description :

Anti-RAMP1 (extracellular) Antibody (#ARR-021) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize RAMP1 from rat and mouse samples. The antibody does not recognize RAMP1 from human samples.

Negative Control :

BLP-RR021

Positive Control :

Synonyms :

Receptor activity-modifying protein 1, Calcitonin receptor-like receptor activity-modifying protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RAMP1

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage