Anti-RXFP2 (extracellular) Antibody | Alomone Labs ARR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-RXFP2 (extracellular) Antibody

catalog :

ARR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Chelakkot V, Liu K, Yoshioka E, Saha S, Xu D, Licursi M, et al. MEK reduces cancer-specific PpIX accumulation through the RSK-ABCB1 and HIF-1α-FECH axes. Sci Rep. 2020;10:22124 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 5), rat testis (lanes 2 and 6), mouse brain (lanes 3 and 7) and mouse testis (lanes 4 and 8): - 1-4. Anti-RXFP2 (extracellular) Antibody (#ARR-012), (1:400).5-8. Anti-RXFP2 (extracellular) Antibody, preincubated with RXFP2 (extracellular) Blocking Peptide (#BLP-RR012).

product information

CAT :

ARR-012

SKU :

ARR-012-CF_0.2 ml

Product Name :

Anti-RXFP2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q91ZZ5

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-RR012

Homology :

Rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CFPLHYDQAEDFGSR, corresponding to amino acid residues 556-570 of mouse RXFP2

Product Page - Scientific background :

Relaxin (RXFP) peptides are members of a family of peptide hormones, structurally related to insulin, which act on a group of four G-protein-coupled receptors known as Relaxin family peptide receptors (RXFPs): RXFP1-4.The RXFP2 (also called LGR8) structure contains 7-transmemebrane spanning domains, at least two binding sites: a long extracellular sequence with a high affinity site containing 10 leucine-rich repeat region and a lower-affinity site in an extracellular loop of a transmembrane region. In addition, the receptor contains an N-terminal ectodomain that acts as a site of primary ligand binding1,2. RXFP2 is also the native receptor for insulin-like peptide 3 (INSL3)1,2. Activation of the receptor leads to increased levels of cAMP.In human, RXFP2 can be detected in the uterus, testis, kidney, brain, thyroid, muscle, peripheral blood cells and bone marrow. Additional expression in rat and mouse was detected in the ovary and gubernaculums. Studies show that activators of RXFP2 may play an important role in the treatment of cryptorchidism and infertility and RXFP2 inhibitors could be used as a means of contraception1. RXFP2 also was found to cause the failure of gubernacular development during embryogenesis in human and mouse3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Relaxin receptor 2, G-protein coupled receptor affecting testicular descent, Leucine-rich repeat-containing G-protein coupled receptor 8, GPR106, GREAT, LGR8, INSL3R

Short Description :

A Rabbit Polyclonal Antibody to Relaxin Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Relaxin receptor 2. Anti-RXFP2 (extracellular) Antibody (#ARR-012) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize Relaxin receptor 2 from mouse and rat samples. The antibody is unlikely to recognize human RXFP2.

Negative Control :

BLP-RR012

Positive Control :

Synonyms :

Relaxin receptor 2, G-protein coupled receptor affecting testicular descent, Leucine-rich repeat-containing G-protein coupled receptor 8, GPR106, GREAT, LGR8, INSL3R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RXFP2

AB Specifictiy :

Not recommended for use on human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage