Anti-Ryanodine Receptor 2 Antibody | Alomone Labs ARR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ryanodine Receptor 2 Antibody

catalog :

ARR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Reference
Guo W, Wei J, Estillore J, Zhang L, Wang R, Sun B, et al. RyR2 disease mutations at the C-terminal domain intersubunit interface alter closed-state stability and channel activation. J Biol Chem. 2021;297:100808 pubmed publisher
Wang Z, Tapa S, Francis Stuart S, Wang L, Bossuyt J, Delisle B, et al. Aging Disrupts Normal Time-of-Day Variation in Cardiac Electrophysiology. Circ Arrhythm Electrophysiol. 2020;13:e008093 pubmed publisher
Thakore P, Pritchard H, Griffin C, Yamasaki E, Drumm B, Lane C, et al. TRPML1 channels initiate Ca2+ sparks in vascular smooth muscle cells. Sci Signal. 2020;13: pubmed publisher
Hamilton S, Terentyeva R, Martin B, Perger F, Li J, Stepanov A, et al. Increased RyR2 activity is exacerbated by calcium leak-induced mitochondrial ROS. Basic Res Cardiol. 2020;115:38 pubmed publisher
Song R, Hu X, Romero M, Holguin M, Kagabo W, Xiao D, et al. Ryanodine receptor subtypes regulate Ca2+ sparks/spontaneous transient outward currents and myogenic tone of uterine arteries in pregnancy. Cardiovasc Res. 2021;117:792-804 pubmed publisher
Boerman E, Segal S. Aging alters spontaneous and neurotransmitter-mediated Ca2+ signaling in smooth muscle cells of mouse mesenteric arteries. Microcirculation. 2020;27:e12607 pubmed publisher
Prajapati C, Pölönen R, Aalto Setala K. Simultaneous recordings of action potentials and calcium transients from human induced pluripotent stem cell derived cardiomyocytes. Biol Open. 2018;7: pubmed publisher
Yu H, Yuan C, Westenbroek R, Catterall W. The AKAP Cypher/Zasp contributes to β-adrenergic/PKA stimulation of cardiac CaV1.2 calcium channels. J Gen Physiol. 2018;150:883-889 pubmed publisher
Wu Y, Chen C, Chien C, Lai H, Jiang S, Chen Y, et al. The type VI adenylyl cyclase protects cardiomyocytes from ?-adrenergic stress by a PKA/STAT3-dependent pathway. J Biomed Sci. 2017;24:68 pubmed publisher
Yang L, Dai D, Yuan C, Westenbroek R, Yu H, West N, et al. Loss of ?-adrenergic-stimulated phosphorylation of CaV1.2 channels on Ser1700 leads to heart failure. Proc Natl Acad Sci U S A. 2016;113:E7976-E7985 pubmed
Forostyak O, Butenko O, Anderova M, Forostyak S, Sykova E, Verkhratsky A, et al. Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells. Stem Cell Res. 2016;16:622-34 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:200).2. Anti-Ryanodine Receptor 2 Antibody, preincubated with Ryanodine Receptor 2 Blocking Peptide (#BLP-RR002).

image 2 :

Expression of RyR2 in rat cardiac muscle - Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of RyR2 in rat cardiac muscle - Immunohistochemical staining of paraffin-embedded sections of ratmyocardiumusingAnti-Ryanodine Receptor 2Antibody (#ARR-002) (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.

product information

CAT :

ARR-002

SKU :

ARR-002-CF_0.2 ml

Product Name :

Anti-Ryanodine Receptor 2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q92736

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-RR002

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human RyR2

Product Page - Scientific background :

It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1); the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain.1-4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

RyR2, Cardiac muscle ryanodine receptor

Short Description :

A Rabbit Polyclonal Antibody to Ryanodine Receptor 2

Long Description :

Anti-Ryanodine Receptor 2 Antibody (#ARR-002) is a highly specific antibody directed against an epitope of human RyR2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR2 from human, rat, and mouse samples.

Negative Control :

BLP-RR002

Positive Control :

Synonyms :

RyR2, Cardiac muscle ryanodine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RYR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage