Anti-Ryanodine Receptor 1 Antibody | Alomone Labs ARR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ryanodine Receptor 1 Antibody

catalog :

ARR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Wang L, Lei Q, Zhao S, Xu W, Dong W, Ran J, et al. Ginkgolide B Maintains Calcium Homeostasis in Hypoxic Hippocampal Neurons by Inhibiting Calcium Influx and Intracellular Calcium Release. Front Cell Neurosci. 2020;14:627846 pubmed publisher
Boerman E, Segal S. Aging alters spontaneous and neurotransmitter-mediated Ca2+ signaling in smooth muscle cells of mouse mesenteric arteries. Microcirculation. 2020;27:e12607 pubmed publisher
García Castañeda M, Vega A, Rodríguez R, Montiel Jaen M, Cisneros B, Zarain Herzberg A, et al. Functional impact of an oculopharyngeal muscular dystrophy mutation in PABPN1. J Physiol. 2017;595:4167-4187 pubmed publisher
Forostyak O, Butenko O, Anderova M, Forostyak S, Sykova E, Verkhratsky A, et al. Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells. Stem Cell Res. 2016;16:622-34 pubmed publisher

image

image 1 :

Western blot analysis of rat skeletal muscle lysates: - 1. Anti-Ryanodine Receptor 1 Antibody (#ARR-001), (1:200). 2. Anti-Ryanodine Receptor 1 Antibody, preincubated with Ryanodine Receptor 1 Blocking Peptide (#BLP-RR001).

image 2 :

Expression of RyR1 in rat skeletal muscle - Immunohistochemical staining of paraffin embedded sections of rat quadriceps using Anti-Ryanodine Receptor 1 Antibody (#ARR-001), (1:100). RyR1 is expressed in fibers of striate muscle. Note that smooth muscle in the arterial walls is negative as well as the surrounding connective tissue. Hematoxilin is used as the counterstain.

image 3 :

Expression of RyR1 in rat skeletal muscle - Immunohistochemical staining of paraffin embedded sections of ratquadricepsusingAnti-Ryanodine Receptor 1Antibody (#ARR-001) (1:100).RyR1is expressed in fibers of striate muscle. Note that smooth muscle in the arterial walls is negative as well as the surrounding connective tissue.Hematoxilin is used as the counterstain.

product information

CAT :

ARR-001

SKU :

ARR-001-CF_0.2 ml

Product Name :

Anti-Ryanodine Receptor 1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21817

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-RR001

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human RyR1

Product Page - Scientific background :

It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1); the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR1 is expressed predominantly in skeletal muscle and in areas of the brain; human RyR1 has at least three known alternative splice variants.Several diseases are attributed to mutations in the RyR1 gene: Central core disease (CCD), multi-minicore disease (MmD) and Malignant hyperthermia (MH) 4.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

RyR1, Skeletal muscle-type ryanodine receptor

Short Description :

A Rabbit Polyclonal Antibody to Ryanodine Receptor 1

Long Description :

Anti-Ryanodine Receptor 1 Antibody (#ARR-001) is a highly specific antibody directed against an epitope of human RyR1. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR1 from human, rat, and mouse samples.

Negative Control :

BLP-RR001

Positive Control :

Synonyms :

RyR1, Skeletal muscle-type ryanodine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RYR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage