Anti-Aquaporin 4 (AQP4) (300-314) Antibody | Alomone Labs AQP-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 4 (AQP4) (300-314) Antibody

catalog :

AQP-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Reference
Khorooshi R, Marczynska J, Dubik M, Dieu R, S xf8 rensen S, Montañana Rosell R, et al. The protective effect of Angiotensin AT2-receptor stimulation in Neuromyelitis optica spectrum disorder is independent of astrocyte-derived BDNF. Mult Scler Relat Disord. 2021;53:103033 pubmed publisher
Duhaut D, Heurteaux C, Gandin C, Ichai C, Quintard H. The Antiedematous Effect of Exogenous Lactate Therapy in Traumatic Brain Injury: A Physiological and Mechanistic Approach. Neurocrit Care. 2021;35:747-755 pubmed publisher
Veys L, Van Houcke J, Aerts J, Van Pottelberge S, Mahieu M, Coens A, et al. Absence of Uptake and Prion-Like Spreading of Alpha-Synuclein and Tau After Intravitreal Injection of Preformed Fibrils. Front Aging Neurosci. 2020;12:614587 pubmed publisher
Hasegawa K, Yamaguchi Y, Tanaka M. Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise. Physiol Rep. 2021;9:e14670 pubmed publisher
Wlodarczyk A, Khorooshi R, Marczynska J, Holtman I, Burton M, Jensen K, et al. Type I interferon-activated microglia are critical for neuromyelitis optica pathology. Glia. 2021;69:943-953 pubmed publisher
Robert J, Weilinger N, Cao L, Cataldi S, Button E, Stukas S, et al. An in vitro bioengineered model of the human arterial neurovascular unit to study neurodegenerative diseases. Mol Neurodegener. 2020;15:70 pubmed publisher
Krueger M, Mages B, Hobusch C, Michalski D. Endothelial edema precedes blood-brain barrier breakdown in early time points after experimental focal cerebral ischemia. Acta Neuropathol Commun. 2019;7:17 pubmed publisher
Kokona D, Ebneter A, Escher P, Zinkernagel M. Colony-stimulating factor 1 receptor inhibition prevents disruption of the blood-retina barrier during chronic inflammation. J Neuroinflammation. 2018;15:340 pubmed publisher
Hardt S, Valek L, Zeng Brouwers J, Wilken Schmitz A, Schaefer L, Tegeder I. Progranulin Deficient Mice Develop Nephrogenic Diabetes Insipidus. Aging Dis. 2018;9:817-830 pubmed publisher
Gu R, Ding X, Tang W, Lei B, Jiang C, Xu G. A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal MÃ¼ller Cell Gliosis. Front Pharmacol. 2018;9:331 pubmed publisher
Dai J, Lin W, Zheng M, Liu Q, He B, Luo C, et al. Alterations in AQP4 expression and polarization in the course of motor neuron degeneration in SOD1G93A mice. Mol Med Rep. 2017;16:1739-1746 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200).3,4. Anti-Aquaporin 4 (AQP4) (300-314) Antibody, preincubated with Aquaporin 4/AQP4 (300-314) Blocking Peptide (#BLP-QP014).

image 2 :

Expression of Aquaporin 4 in rat brain - Immunohistochemical staining of rat brain using Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200). A. Aquaporin 4 (green) is detected in blood vessels in layers 1-3 of rat neocortex. B. Astrocytic processes were stained using mouse anti glial fibrillary acidic protein (GFAP) (red) and DAPI (blue) was used to delineate the cortical layers. C. Merge of Aquaporin 4 and GFAP shows overlap along blood vessels (arrows) in agreement with the role of astrocytes and Aquaporin 4 in regulation of the blood-brain barrier.

image 3 :

Expression of Aquaporin 4 in rat brain - Immunohistochemical staining of rat brain usingAnti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014) (1:200). A. Aquaporin 4 (green) is detected in blood vessels in layers 1-3 of rat neocortex. B. Astrocytic processes were stained using mouse anti glial fibrillary acidic protein (GFAP) (red) and DAPI (blue) was used to delineate the cortical layers. C. Merge of Aquaporin 4 and GFAP shows overlap along blood vessels (arrows) in agreement with the role of astrocytes and Aquaporin 4 in regulation of the blood-brain barrier.

product information

CAT :

AQP-014

SKU :

AQP-014-CF_0.2 ml

Product Name :

Anti-Aquaporin 4 (AQP4) (300-314) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47863

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP014

Homology :

Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4

Product Page - Scientific background :

Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

WCH4, Mercurial-insensitive water channel

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 4 Channel

Long Description :

Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014) is a highly specific antibody directed against an epitope of rat AQP4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize the Aquaporin 4 channel from rat, mouse, and human samples.

Negative Control :

BLP-QP014

Positive Control :

Synonyms :

WCH4, Mercurial-insensitive water channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage