Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody | Alomone Labs AQP-014-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody

catalog :

AQP-014-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1, 2. Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014-GP), (1:400).3, 4. Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody, preincubated with Aquaporin 4 (AQP4) (300-314) Blocking Peptide (#BLP-QP014).

image 2 :

Expression of Aquaporin 4 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014-GP), (1:200), followed by goat anti-guinea pig - Alexa Fluor-594. A. Staining in the hippocampal CA1 region, showed immunoreactivity (red) in blood vessel outlines (arrows). B. Pre-incubation of the antibody with Aquaporin 4 (AQP4) (300-314) Blocking Peptide (#BLP-QP014), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.

product information

CAT :

AQP-014-GP

SKU :

AQP-014-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47863

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-QP014

Homology :

Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4

Product Page - Scientific background :

Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

WCH4, Mercurial-insensitive water channel, MIWC

Short Description :

A Guinea Pig Polyclonal Antibody to Aquaporin 4 (AQP4)

Long Description :

Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Aquaporin 4 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Aquaporin 4 (AQP4) (300-314) (#AQP-014) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-QP014

Positive Control :

Synonyms :

WCH4, Mercurial-insensitive water channel, MIWC

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200-1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage