Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody | Alomone Labs AQP-014-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody

catalog :

AQP-014-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Tradtrantip L, Yeaman M, Verkman A. Cytoprotective IgG antibodies in sera from a subset of patients with AQP4-IgG seropositive neuromyelitis optica spectrum disorder. Sci Rep. 2021;11:21962 pubmed publisher

image

image 1 :

Expression of Aquaporin 4 in rat and mouse brain - Immunohistochemical staining of Aquaporin 4 in rat and mouse brain free floating frozen sections using Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (#AQP-014-AR), (1:80). A. AQP4 staining (red) in rat hippocampal CA1 region appears in outlines of blood vessels (arrows). DAPI is used as the counterstain (blue) and stains the pyramidal layer (P). B. AQP4 staining (red) in mouse hippocampal dentate gyrus region appears also in outlines of blood vessels (arrows). DAPI is used as the counterstain (blue) and stains the granule layer (G).

product information

CAT :

AQP-014-AR

SKU :

AQP-014-AR_50 mcl

Product Name :

Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47863

Applications :

IF IHC

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP014

Homology :

Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4

Product Page - Scientific background :

Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

WCH4, Mercurial-insensitive water channel

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014) is a highly specific antibody directed against an epitope of rat AQP4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize the Aquaporin 4 channel from rat, mouse, and human samples. Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (#AQP-014-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-QP014

Positive Control :

Synonyms :

WCH4, Mercurial-insensitive water channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AQP-014), and immunohistochemical staining (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:80-1:100

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage