Anti-Aquaporin 11 (extracellular) Antibody | Alomone Labs AQP-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 11 (extracellular) Antibody

catalog :

AQP-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Aquaporin 11 (extracellular) Antibody (#AQP-011), (1:1000).3,4. Anti-Aquaporin 11 (extracellular) Antibody, preincubated with Aquaporin 11 (extracellular) Blocking Peptide (#BLP-QP011).

image 2 :

Western blot analysis of rat liver lysate: - 1.Anti-Aquaporin 11 (extracellular)Antibody (#AQP-011) (1:1000).2. Anti-Aquaporin 11 (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)SLSLTKYHFDER corresponding to amino acid residues 140-151 of rat AQP11 (AccessionQ8CHM1). 2ndextracellular loop.

product information

CAT :

AQP-011

SKU :

AQP-011-CF_0.2 ml

Product Name :

Anti-Aquaporin 11 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CHM1

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP011

Homology :

Mouse - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SLSLTKYHFDER, corresponding to amino acid residues 140-151 of rat AQP11

Product Page - Scientific background :

The aquaporins (AQP), also known as the major intrinsic protein superfamily (MIP), are a family of integral membrane proteins. In mammals, 13 aquaporins were described (AQP0-AQP12). Aquaporin 11 (AQP11) is a newly described member of the aquaporins family. Aquaporin 11 (AQP11) has been identified with unusual pore-forming NPA (asparagine-proline-alanine) boxes. AQP11 presents a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1. There are two subfamilies: the aquaporins, which transport only water and the aquaglyceroporins, which transport glycerol, urea, and other small solutes in addition to water2.Aquaporins and aquaglyceroporins are ubiquitously expressed, including in the plasma membrane of renal epithelia, brain astrocytes, and red blood cells3. AQP11 is expressed in several tissues including testis, kidney, liver, and brain. In the brain, AQP11 is found in Purkinje cells, hippocampal neurons of CA1 and CA2, and cerebral cortical neurons4. In the kidney AQP11 has a central role in vesicular homeostasis as demonstrated in experiments using AQP11 knockout mice which display growth retardation and progressively die before weaning. The cause of death seems to be advanced renal failure5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

AQP11

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 11 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Aquaporin 11. Anti-Aquaporin 11 (extracellular) Antibody (#AQP-011) can be used in western blot analysis. It has been designed to recognize AQP11 from rat and mouse samples.

Negative Control :

BLP-QP011

Positive Control :

Synonyms :

AQP11

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP11

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage