Anti-Aquaporin 9 Antibody | Alomone Labs AQP-009 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 9 Antibody

catalog :

AQP-009

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, western blot knockout validation, immunohistochemistry knockout validation

more info or order :

Alomone Labs product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 2e western blot knockout validation; mouse; 1:500; loading ...; fig 2f	Cheng Q, Ding H, Fang J, Fang X, Liu H, Wang J, et al. Aquaporin 9 Represents a Novel Target of Chronic Liver Injury That May Antagonize Its Progression by Reducing Lipotoxicity. Oxid Med Cell Longev. 2021;2021:5653700 pubmed publisher
	Chiadak J, Gena P, Gregoire F, Bolaky N, Delforge V, Perret J, et al. Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes. Int J Mol Sci. 2017;18: pubmed publisher
	Sales A, Duarte A, Santos R, Alves K, Lima L, Rodrigues G, et al. Modulation of aquaporins 3 and 9 after exposure of ovine ovarian tissue to cryoprotectants followed by in vitro culture. Cell Tissue Res. 2016;365:415-24 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes: - 1,2,3. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).4,5,6. Anti-Aquaporin 9 Antibody, preincubated with Aquaporin 9 Blocking Peptide (#BLP-QP009).

image 2 :

Expression of AQP9 in rat liver - Immunohistochemical staining of rat liver paraffin embedded sections using Anti-Aquaporin 9 Antibody (#AQP-009) (1:100). AQP9 (brown) is expressed in hepatocytes of the liver parenchyma. Hematoxilin is used as the counterstain.

image 3 :

Peptide (C)EKDGAKKSLMQRLALK corresponding to amino acid residues 4-19 of rat AQP9 (Accession P56627).Intracellular N-terminus.

product information

CAT :

AQP-009

SKU :

AQP-009-CF_0.2 ml

Product Name :

Anti-Aquaporin 9 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56627

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP009

Homology :

Mouse - 13/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP9

Product Page - Scientific background :

Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water (AQP-1, AQP-2, AQP4, AQP-5, AQP-6, AQP-8), and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2AQP-9 is expressed in several tissues including the brain, liver, testis and spleen. In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

AQP9, Aquaglyceroporin-9

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 9 Channel

Long Description :

Anti-Aquaporin 9 Antibody (#AQP-009) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP9 from mouse and rat samples.

Negative Control :

BLP-QP009

Positive Control :

Synonyms :

AQP9, Aquaglyceroporin-9

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP9

AB Specifictiy :

Unlikely to recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage