Anti-Aquaporin 8 Antibody | Alomone Labs AQP-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 8 Antibody

catalog :

AQP-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Wang X, Yuan X, Su Y, Hu J, Ji Q, Fu S, et al. Targeting Purinergic Receptor P2RX1 Modulates Intestinal Microbiota and Alleviates Inflammation in Colitis. Front Immunol. 2021;12:696766 pubmed publisher
Bracho G, Altamirano G, Kass L, Luque E, Bosquiazzo V. Hyperandrogenism Induces Histo-Architectural Changes in the Rat Uterus. Reprod Sci. 2019;26:657-668 pubmed publisher

image

image 1 :

Western blot analysis of rat small intestine membranes: - 1. Anti-Aquaporin 8 Antibody (#AQP-008), (1:200).2. Anti-Aquaporin 8 Antibody, preincubated with Aquaporin 8 Blocking Peptide (#BLP-QP008).

image 2 :

Expression of AQP8 in rat pancreas - Immunohistochemical staining of rat pancreas paraffin-embedded sections using Anti-Aquaporin 8 Antibody (#AQP-008), (1:100). Staining (brown) is specific for the apical portion of acinar cells in the exocrine pancreas. Hematoxilin is used as the counterstain.

image 3 :

Western blot analysis of rat small intestine membranes: - 1.Anti-Aquaporin 8Antibody (#AQP-008) (1:200).2.Anti-Aquaporin 8 Antibody preincubated with the control peptide antigen.

product information

CAT :

AQP-008

SKU :

AQP-008-CF_0.2 ml

Product Name :

Anti-Aquaporin 8 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56405

Applications :

IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP008

Homology :

Unlikely to recognize mouse or human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)REIKGKETNMADSYH, corresponding to amino acid residues 14-28 of rat AQP8

Product Page - Scientific background :

Aquaporin 8 (AQP-8) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include besides AQP-8, AQP-1, AQP-2, AQP4 and AQP-5, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.2AQP-8 is expressed along the gastrointestinal tract including small intestine, colon, pancreas and liver and also in other organs such as testis, lung and kidney.The physiological function of AQP-8 is not entirely clear but studies suggest that it has an important role in colonic water adsorption and hepatocyte bile formation.3,4 Interestingly, AQP-8 expression is markedly altered in gastrointestinal disorders such as ulcerative colitis and inflammatory bowel disease.5

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

AQP8

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 8 Channel

Long Description :

Anti-Aquaporin 8 Antibody (#AQP-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP8 from rat samples only.

Negative Control :

BLP-QP008

Positive Control :

Synonyms :

AQP8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage