Anti-Aquaporin 7 Antibody | Alomone Labs AQP-007 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 7 Antibody

catalog :

AQP-007

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Ziemens A, Sonntag S, Wulfmeyer V, Edemir B, Bleich M, Himmerkus N. Claudin 19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells. Int J Mol Sci. 2019;20: pubmed publisher
Chung S, Kim S, Son M, Kim M, Koh E, Shin S, et al. Empagliflozin Contributes to Polyuria via Regulation of Sodium Transporters and Water Channels in Diabetic Rat Kidneys. Front Physiol. 2019;10:271 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney membranes: - 1,2. Anti-Aquaporin 7 Antibody, (#AQP-007), (1:500).3,4. Anti-Aquaporin 7 Antibody, preincubated with Aquaporin 7 Blocking Peptide (#BLP-QP007).

image 2 :

Expression of AQP7 in rat kidney - Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 7 Antibody (#AQP-007), (1:100). A. In the minor calyx, AQP7 staining (deep red) is specific for epithelial cells of the uroepithelium B. In the cortical labyrinth, AQP7 staining is specific for renal tubules while the stronger staining is evident in the brush border (blue arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of AQP7 in rat kidney - Immunohistochemical staining of paraffin-embedded rat kidney sections usingAnti-Aquaporin 7 Antibody (#AQP-007) (1:100). A. In the minor calyx AQP7 staining (deep red) is specific for epithelial cells of the uroepithelium B. In the cortical labyrinth AQP7 staining is specific for renal tubules while the stronger staining is evident in the brush border (blue arrows). Hematoxilin is used as the counterstain.

product information

CAT :

AQP-007

SKU :

AQP-007-CF_0.2 ml

Product Name :

Anti-Aquaporin 7 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56403

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP007

Homology :

Mouse - 15/18 amino acid residues identical; human - 11/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

ENIQSVLQKTWVREFLAE, corresponding to amino acid residues 7-24 of rat AQP7

Product Page - Scientific background :

Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.AQP-7 is expressed in ovary, testis, kidney and adipose tissue.The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and Type 2 Diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 7 Channel

Long Description :

Anti-Aquaporin 7 Antibody (#AQP-007) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize AQP-7 from mouse and rat samples, and may recognize the channel from human samples.

Negative Control :

BLP-QP007

Positive Control :

Synonyms :

AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage