Anti-Aquaporin 6 Antibody | Alomone Labs AQP-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 6 Antibody

catalog :

AQP-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-Aquaporin 6 Antibody (#AQP-006), (1:200).2. Anti-Aquaporin 6 Antibody, preincubated with Aquaporin 6 Blocking Peptide (#BLP-QP006).

image 2 :

Western blot analysisof rat kidney membranes: - 1.Anti-Aquaporin 6Antibody (#AQP-006) (1:200).2. Anti-Aquaporin 6 Antibodypreincubated with the control peptide antigen.

image 3 :

Cell surface detection of AT1receptor in live intact human THP-1 monocytic leukemia cells: - ___ Cells alone.___ Cells + Rabbit IgG isotype control-FITC.___ Cells + Angiotensin IIReceptor Type-1 (extracellular)-FITCAntibody (#AAR-011-F) (5 g).

product information

CAT :

AQP-006

SKU :

AQP-006-CF_0.2 ml

Product Name :

Anti-Aquaporin 6 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WTY0

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP006

Homology :

Mouse - 15/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EPQKKESQTNSEDTEVSV corresponding to amino acid residues 259-276 of rat AQP6

Product Page - Scientific background :

It has been long known that water must cross biological membranes by means other than simple diffusion. However, it was not until recently, with the discovery of the Aquaporin 1 water channel that this question was answered. The importance of this discovery was underlined by the awarding, in 2003, the Nobel Prize in Chemistry to Peter Agre for the discovery of water channels. Today, eleven mammalian proteins that belong to the Aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini.Aquaporin 6 (AQP6) appears to be exclusively expressed in the kidney, particularly in intracellular vesicles in collecting duct intercalated cells. Unlike the other aquaporins, AQP6 was found to allow permeation of anions following activation with acidic pH or Hg2+ ions. Increased expression of AQP6 was shown in models of chronic alkalosis and lithium-induced nephrogenic diabetes insipidus.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

AQP6, AQP2L, Kidney-specific aquaporin

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 6 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat AQP6. Anti-Aquaporin 6 Antibody (#AQP-006) can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP6 from mouse and rat samples.

Negative Control :

BLP-QP006

Positive Control :

Synonyms :

AQP6, AQP2L, Kidney-specific aquaporin

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage