product summary
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company name :
Alomone Labs
product type :
antibody
product name :
Anti-Aquaporin 5 Antibody
catalog :
AQP-005
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
NA
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
Alomone Labs product webpage
citations: 34
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; fig 3
Maimets M, Rocchi C, Bron R, Pringle S, Kuipers J, Giepmans B, et al. Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals. Stem Cell Reports. 2016;6:150-62 pubmed publisher
Yasuhara R, Kang S, Irie T, Mabuchi Y, Kujiraoka S, Yukimori A, et al. Role of Snai2 and Notch signaling in salivary gland myoepithelial cell fate. Lab Invest. 2022;: pubmed publisher
Mo Y, Nakamura H, Tanaka T, Odani T, Perez P, Ji Y, et al. Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome. J Clin Invest. 2022;132: pubmed publisher
Zhou B, Stueve T, Mihalakakos E, Miao L, Mullen D, Wang Y, et al. Comprehensive epigenomic profiling of human alveolar epithelial differentiation identifies key epigenetic states and transcription factor co-regulatory networks for maintenance of distal lung identity. BMC Genomics. 2021;22:906 pubmed publisher
Kim J, Lee S, Park K. CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands. Int J Mol Sci. 2021;22: pubmed publisher
Peng X, Wu Y, Brouwer U, van Vliet T, Wang B, Demaria M, et al. Cellular senescence contributes to radiation-induced hyposalivation by affecting the stem/progenitor cell niche. Cell Death Dis. 2020;11:854 pubmed publisher
Takashi Y, Tomita K, Kuwahara Y, Roudkenar M, Roushandeh A, Igarashi K, et al. Mitochondrial dysfunction promotes aquaporin expression that controls hydrogen peroxide permeability and ferroptosis. Free Radic Biol Med. 2020;161:60-70 pubmed publisher
Weber J, Rajan S, Schremmer C, Chao Y, Krasteva Christ G, Kannler M, et al. TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema. JCI Insight. 2020;5: pubmed publisher
Dall Aglio C, Mercati F, De Felice E, Tardella F, Kamphues J, Cappai M, et al. Influence of Different Feed Physical Forms on Mandibular Gland in Growing Pigs. Animals (Basel). 2020;10: pubmed publisher
Kaihoko Y, Tsugami Y, Suzuki N, Suzuki T, Nishimura T, Kobayashi K. Distinct expression patterns of aquaporin 3 and 5 in ductal and alveolar epithelial cells in mouse mammary glands before and after parturition. Cell Tissue Res. 2020;380:513-526 pubmed publisher
Kim J, Kim J, Choi M, Kim S, Kim Y, Choi J. Adipose-derived mesenchymal stem cells regenerate radioiodine-induced salivary gland damage in a murine model. Sci Rep. 2019;9:15752 pubmed publisher
Suzuki A, Shim J, Ogata K, Yoshioka H, Iwata J. Cholesterol metabolism plays a crucial role in the regulation of autophagy for cell differentiation of granular convoluted tubules in male mouse submandibular glands. Development. 2019;146: pubmed publisher
Thiemann R, Nelson D, Michael DiPersio C, Larsen M, LaFlamme S. Establishment of a Murine Pro-acinar Cell Line to Characterize Roles for FGF2 and α3β1 Integrins in Regulating Pro-acinar Characteristics. Sci Rep. 2019;9:10984 pubmed publisher
Vining K, Lombaert I, Patel V, Kibbey S, Pradhan Bhatt S, Witt R, et al. Neurturin-containing laminin matrices support innervated branching epithelium from adult epithelial salispheres. Biomaterials. 2019;216:119245 pubmed publisher
Tanaka J, Mabuchi Y, Hata K, Yasuhara R, Takamatsu K, Kujiraoka S, et al. Sox9 regulates the luminal stem/progenitor cell properties of salivary glands. Exp Cell Res. 2019;: pubmed publisher
Shitara A, Malec L, Ebrahim S, Chen D, Bleck C, Hoffman M, et al. Cdc42 negatively regulates endocytosis during apical membrane maintenance in live animals. Mol Biol Cell. 2019;30:324-332 pubmed publisher
Hosseini Z, Nelson D, Moskwa N, Larsen M. Generating Embryonic Salivary Gland Organoids. Curr Protoc Cell Biol. 2019;83:e76 pubmed publisher
Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, et al. Generation of orthotopically functional salivary gland from embryonic stem cells. Nat Commun. 2018;9:4216 pubmed publisher
Song E, Min S, Oyelakin A, Smalley K, Bard J, Liao L, et al. Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance. Sci Rep. 2018;8:14043 pubmed publisher
Shi Z, Ye W, Zhang J, Zhang F, Yu D, Yu H, et al. LipoxinA4 attenuates acute pancreatitis-associated acute lung injury by regulating AQP-5 and MMP-9 expression, anti-apoptosis and PKC/SSeCKS-mediated F-actin activation. Mol Immunol. 2018;103:78-88 pubmed publisher
Shin H, Lee S, Hong H, Lim Y, Koh W, Lim J. Stem cell properties of human clonal salivary gland stem cells are enhanced by three-dimensional priming culture in nanofibrous microwells. Stem Cell Res Ther. 2018;9:74 pubmed publisher
Zhou B, Flodby P, Luo J, Castillo D, Liu Y, Yu F, et al. Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. J Clin Invest. 2018;128:970-984 pubmed publisher
Hosseini Z, Nelson D, Moskwa N, Sfakis L, Castracane J, Larsen M. FGF2-dependent mesenchyme and laminin-111 are niche factors in salivary gland organoids. J Cell Sci. 2018;131: pubmed publisher
Farrell H, Bruce K, Lawler C, Oliveira M, Cardin R, Davis Poynter N, et al. Murine Cytomegalovirus Spreads by Dendritic Cell Recirculation. MBio. 2017;8: pubmed publisher
Kida H, Takahashi T, Nakamura Y, Kinoshita T, Hara M, Okamoto M, et al. Pathogenesis of Lethal Aspiration Pneumonia in Mecp2-null Mouse Model for Rett Syndrome. Sci Rep. 2017;7:12032 pubmed publisher
Sakai M, Matsushita T, Hoshino R, Ono H, Ikai K, Sakai T. Identification of the protective mechanisms of Lactoferrin in the irradiated salivary gland. Sci Rep. 2017;7:9753 pubmed publisher
Bärnthaler T, Maric J, Platzer W, Konya V, Theiler A, Hasenöhrl C, et al. The Role of PGE2 in Alveolar Epithelial and Lung Microvascular Endothelial Crosstalk. Sci Rep. 2017;7:7923 pubmed publisher
Zeng M, Szymczak M, Ahuja M, Zheng C, Yin H, Swaim W, et al. Restoration of CFTR Activity in Ducts Rescues Acinar Cell Function and Reduces Inflammation in Pancreatic and Salivary Glands of Mice. Gastroenterology. 2017;153:1148-1159 pubmed publisher
Flodby P, Li C, Liu Y, Wang H, Rieger M, Minoo P, et al. Cell-specific expression of aquaporin-5 (Aqp5) in alveolar epithelium is directed by GATA6/Sp1 via histone acetylation. Sci Rep. 2017;7:3473 pubmed publisher
Rossi L, Nicoletti M, Carmosino M, Mastrofrancesco L, Di Franco A, Indrio F, et al. Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy. J Diabetes Res. 2017;2017:4360357 pubmed publisher
Choi J, Shin H, An H, Kim Y, Lim J. Radioprotective effects of Keratinocyte Growth Factor-1 against irradiation-induced salivary gland hypofunction. Oncotarget. 2017;8:13496-13508 pubmed publisher
Marconett C, Zhou B, Sunohara M, Pouldar T, Wang H, Liu Y, et al. Cross-Species Transcriptome Profiling Identifies New Alveolar Epithelial Type I Cell-Specific Genes. Am J Respir Cell Mol Biol. 2017;56:310-321 pubmed publisher
Joo E, Lombaert I, Yamada K. Hyperacetylation of Microtubules in Mesenchymal Cells Increases Cytokeratin 14-Positive Epithelial Progenitors in Developing Salivary Glands. J Dent Res. 2016;95:1518-1527 pubmed
Liebler J, Marconett C, Juul N, Wang H, Liu Y, Flodby P, et al. Combinations of differentiation markers distinguish subpopulations of alveolar epithelial cells in adult lung. Am J Physiol Lung Cell Mol Physiol. 2016;310:L114-20 pubmed publisher
image
image 1 :
Alomone Labs AQP-005 image 1
Western blot analysis of mouse salivary gland (1 and 3) and rat lung (2 and 4): - 1,2. Anti-Aquaporin 5 Antibody (#AQP-005), (1:200).3,4. Anti-Aquaporin 5 Antibody, preincubated with Aquaporin 5 Blocking Peptide (#BLP-QP005).
image 2 :
Alomone Labs AQP-005 image 2
Expression of AQP5 in rat lung - Immunohistochemical staining of rat lung sections using Anti-Aquaporin 5 Antibody (#AQP-005), (left panel). The strong staining in the alveoli (red brown) is consistent with type 1 pneumocytes (arrows). Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
image 3 :
Alomone Labs AQP-005 image 3
Western blot analysisof mouse salivary gland (1 and 3) and rat lung (2 and 4): - 12.Anti-Aquaporin 5Antibody (#AQP-005) (1:200).34. Anti-Aquaporin 5 Antibody preincubated with the control peptide antigen.
product information
CAT :
AQP-005
SKU :
AQP-005-CF_0.2 ml
Product Name :
Anti-Aquaporin 5 Antibody
Group Type :
Antibodies
Product Type :
Antibodies
Clonality :
Polyclonal
Accession :
P47864
Applications :
IC IF IHC WB
Reactivity :
Human Rat Mouse
Host :
Rabbit
Blocking Peptide :
BLP-QP005
Homology :
Mouse - identical; human - 10/16 amino acid residues identical
Formulation :
PBS pH7.4
isotype :
Rabbit IgG
Peptide confirmation :
Confirmed by amino acid analysis and mass spectrometry
Reconstitution :
0.2 ml double distilled water (DDW).
Antibody Concentration After Reconstitut ... :
1 mg/ml
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preservative :
No Preservative
Immunogen Location :
Intracellular, C-terminus
Label :
Unconjugated
Storage Before Reconstitution :
The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
immunogen source species :
Rat
Sequence :
(C)DHREERKKTIELTAH corresponding to amino acid residues 251-265 of rat AQP5
Product Page - Scientific background :
The importance of the aquaporin water channels was underscored by awarding the 2003 Nobel Prize in Chemistry to Peter Agre "for the discovery of water channels". Today, eleven mammalian proteins that belong to the aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. Aquaporin 5 (AQP5) is expressed in secretory glands, lung and eye, where it works in modulating the rate of fluid release. Since a number of pathological conditions including pulmonary edema, respiratory distress syndrome and congestive heart failure are characterized by disrupted fluid transport, it has been suggested that modulation of the expression of AQP5 could be a novel modality of treatment of the above illnesses. Indeed, several studies have shown that AQP5 expression is downregulated in animal models of acute lung infection.
Applications may also work in :
IC IF IHC WB
Supplier :
Alomone Labs
Target :
AQP5
Short Description :
A Rabbit Polyclonal Antibody to Aquaporin 5 Channel
Long Description :
Anti-Aquaporin 5 Antibody (#AQP-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AQP5 from mouse, rat, and human samples.
Negative Control :
BLP-QP005
Positive Control :
NA
Synonyms :
AQP5
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Specifictiy :
AQP5
Form :
Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Comment :
Contact Alomone Labs for technical support and product customization
Species reactivity key :
H- Human, M- Mouse, R- Rat
Is Toxin :
No
Purity :
Affinity purified on immobilized antigen.
UNSPSC :
41116161
Cited Application :
IHC ICC Whole Tissue Staining
Clone :
NA
Standard quality control of each lot :
Western blot analysis
Antigen preadsorption control :
1 µg peptide per 1 µg antibody
Application Dilutions: Immunohistochemis ... :
Contact Alomone for information
Application Dilutions: Western blot wb :
1:200
more info or order :
Alomone Labs product webpage
company information
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972 2 531 8002
headquarters: Israel