Anti-Aquaporin 3 Antibody | Alomone Labs AQP-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 3 Antibody

catalog :

AQP-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Ikarashi N, Mizukami N, Pei C, Uchino R, Fujisawa I, Fukuda N, et al. Role of Cutaneous Aquaporins in the Development of Xeroderma in Type 2 Diabetes. Biomedicines. 2021;9: pubmed publisher
Hasegawa K, Yamaguchi Y, Tanaka M. Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise. Physiol Rep. 2021;9:e14670 pubmed publisher
Ikarashi N, Kon R, Nagoya C, Ishikura A, Sugiyama Y, Takahashi J, et al. Effect of Astaxanthin on the Expression and Activity of Aquaporin-3 in Skin in an In-Vitro Study. Life (Basel). 2020;10: pubmed publisher
Laszczyk A, Higashi A, Patel S, Johnson C, Soofi A, Abraham S, et al. Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney. J Am Soc Nephrol. 2020;31:1212-1225 pubmed publisher
Chung S, Kim S, Son M, Kim M, Koh E, Shin S, et al. Empagliflozin Contributes to Polyuria via Regulation of Sodium Transporters and Water Channels in Diabetic Rat Kidneys. Front Physiol. 2019;10:271 pubmed publisher
Yu G, Liu Q, Dong X, Tang K, Li B, Liu C, et al. Inhibition of inflammation using diacerein markedly improved renal function in endotoxemic acute kidney injured mice. Cell Mol Biol Lett. 2018;23:38 pubmed publisher
Chen G, Shi Y, Liu M, Sun J. circHIPK3 regulates cell proliferation and migration by sponging miR-124 and regulating AQP3 expression in hepatocellular carcinoma. Cell Death Dis. 2018;9:175 pubmed publisher
Thiagarajah J, Chang J, Goettel J, Verkman A, Lencer W. Aquaporin-3 mediates hydrogen peroxide-dependent responses to environmental stress in colonic epithelia. Proc Natl Acad Sci U S A. 2017;114:568-573 pubmed publisher
Sales A, Duarte A, Santos R, Alves K, Lima L, Rodrigues G, et al. Modulation of aquaporins 3 and 9 after exposure of ovine ovarian tissue to cryoprotectants followed by in vitro culture. Cell Tissue Res. 2016;365:415-24 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-Aquaporin 3 Antibody (#AQP-003), (1:200).2. Anti-Aquaporin 3 Antibody, preincubated with Aquaporin 3 Blocking Peptide (#BLP-QP003).

image 2 :

Expression of Aquaporin 3 in rat colon - Immunohistochemical staining of paraffin embedded longitudinal section of rat colon using Anti-Aquaporin 3 Antibody (#AQP-003), (brown). AQP3 is detected in absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Hematoxilin is used as the counterstain. Mucosa (M), submucosa (SM) and muscularis externa (ME).

product information

CAT :

AQP-003

SKU :

AQP-003-CF_0.2 ml

Product Name :

Anti-Aquaporin 3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47862

Applications :

IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP003

Homology :

Mouse - 17/18 amino acid residues identical; sheep, human - 15/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3

Product Page - Scientific background :

Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow the passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7, AQP-9 and AQP-10.1 Little is known about the function of the two newest members, AQP-11 and AQP-12. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3

Applications may also work in :

IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

AQP3, Aquaglyceroporin-3, GIL, GLIP

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 3 Channel

Long Description :

Anti-Aquaporin 3 Antibody (#AQP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize the AQP3 channel from rat, human, and mouse samples.

Negative Control :

BLP-QP003

Positive Control :

Synonyms :

AQP3, Aquaglyceroporin-3, GIL, GLIP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage