Anti-Aquaporin 3-ATTO Fluor-594 Antibody | Alomone Labs AQP-003-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 3-ATTO Fluor-594 Antibody

catalog :

AQP-003-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ikarashi N, Kaneko M, Fujisawa I, Fukuda N, Yoshida R, Kon R, et al. Wound-Healing and Skin-Moisturizing Effects of Sasa veitchii Extract. Healthcare (Basel). 2021;9: pubmed publisher

image

image 1 :

Expression of Aquaporin 3 in rat colon - Immunohistochemical staining of rat colon paraffin-embedded sections using Anti-Aquaporin 3-ATTO Fluor-594 Antibody (#AQP-003-AR), (1:100). Staining (red) is present in absorptive cells of the colonic epithelium. Hoechst 33342 is used as counterstain (blue).

image 2 :

Expression of Aquaporin 3 in rat kidney - Immunohistochemical staining of rat kidney paraffin-embedded sections usingAnti-Aquaporin 3-ATTO-594 Antibody (#AQP-003-AR) (1:100). A. Staining (red) is present in collecting ducts in the corticomedullary junction. B. High magnification confocal image showing staining in discrete collecting ducts cells. Hoechst 33342 is used as counterstain (blue).

image 3 :

Immuno-colocalization of Calnexin and Presenilin-1 in mouse cortex - Immunohistochemical staining of perfusion-fixed frozen mouse parietal cortex sections using Anti-Presenilin-1-ATTO-488 Antibody (#AIP-011-AG) (1:60) and Anti-Calnexin-ATTO-594 Antibody (#ACS-009-AR) (1:60). A. Calnexin staining (red) appears in neuronal profiles. B. Presenilin-1 staining (green) in the same section appears in neuronal profiles and apical dendrites (arrows). C. Merger of A and B demonstrates colocalization in several neurons (arrows). Cell nuclei are stained using DAPI (blue) as the counterstain. Images were acquired with 25x objective.

product information

CAT :

AQP-003-AR

SKU :

AQP-003-AR_50 mcl

Product Name :

Anti-Aquaporin 3-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47862

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP003

Homology :

Mouse - 17/18 amino acid residues identical; sheep, human - 15/18 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3

Product Page - Scientific background :

Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7, AQP-9 and AQP-10.1 Little is known about the function of the two newest members, AQP-11 and AQP-12. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

AQP3, Aquaglyceroporin-3, GIL, GLIP

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 3 Channel Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-Aquaporin 3 Antibody (#AQP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize the AQP3 channel from rat, human, and mouse samples. Anti-Aquaporin 3-ATTO Fluor-594 Antibody (#AQP-003-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 3-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-QP003

Positive Control :

Synonyms :

AQP3, Aquaglyceroporin-3, GIL, GLIP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC FC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AQP-003), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage