Anti-Aquaporin 2 Antibody | Alomone Labs AQP-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 2 Antibody

catalog :

AQP-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig s3f	Ranea Robles P, Portman K, Bender A, Lee K, He J, Mulholland D, et al. Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice. Kidney360. 2021;2:1441-1454 pubmed publisher
immunohistochemistry; mouse; loading ...; fig s2b	Dvela Levitt M, Kost Alimova M, Emani M, Kohnert E, Thompson R, Sidhom E, et al. Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy. Cell. 2019;178:521-535.e23 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 6f	Katsumata O, Mori M, Sawane Y, Niimura T, Ito A, Okamoto H, et al. Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues. Histochem Cell Biol. 2017;148:577-596 pubmed publisher
	Li S, Hildreth C, Rahman A, Barton S, Wyse B, Lim C, et al. Renal denervation does not affect hypertension or the renin-angiotensin system in a rodent model of juvenile-onset polycystic kidney disease: clinical implications. Sci Rep. 2021;11:14286 pubmed publisher
	Khayyat N, Zaika O, Tomilin V, Pyrshev K, Pochynyuk O. Angiotensin II increases activity of the ClC-K2 Cl- channel in collecting duct intercalated cells by stimulating production of reactive oxygen species. J Biol Chem. 2021;296:100347 pubmed publisher
	Han S, Williams R, Kim M, Heller D, D AGATI V, Schmidt Supprian M, et al. Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury. JCI Insight. 2020;5: pubmed publisher
	Laszczyk A, Higashi A, Patel S, Johnson C, Soofi A, Abraham S, et al. Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney. J Am Soc Nephrol. 2020;31:1212-1225 pubmed publisher
	Chow T, Wong F, Monetti C, Nagy A, Cox B, Rogers I. Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayers. NPJ Regen Med. 2020;5:7 pubmed publisher
	Shi S, Montalbetti N, Wang X, Rush B, Marciszyn A, Baty C, et al. Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel. J Biol Chem. 2020;295:4950-4962 pubmed publisher
	Mazzocchi M, Di Giusto G, Porta M, Pizzoni A, Beltramone N, Ford P, et al. Na+/H+ exchanger isoform 1 activity in AQP2-expressing cells can be either proliferative or anti-proliferative depending on extracellular pH. J Physiol Biochem. 2020;76:37-48 pubmed publisher
	Kumaran G, Hanukoglu I. Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns. FEBS J. 2020;287:1176-1194 pubmed publisher
	Mikoda N, Sonoda H, Oshikawa S, Hoshino Y, Matsuzaki T, Ikeda M. A bell-shaped pattern of urinary aquaporin-2-bearing extracellular vesicle release in an experimental model of nephronophthisis. Physiol Rep. 2019;7:e14092 pubmed publisher
	Chung S, Kim S, Son M, Kim M, Koh E, Shin S, et al. Empagliflozin Contributes to Polyuria via Regulation of Sodium Transporters and Water Channels in Diabetic Rat Kidneys. Front Physiol. 2019;10:271 pubmed publisher
	Tomilin V, Mamenko M, Zaika O, Wingo C, Pochynyuk O. TRPV4 deletion protects against hypokalemia during systemic K+ deficiency. Am J Physiol Renal Physiol. 2019;316:F948-F956 pubmed publisher
	Kim W, Nam S, Choi A, Kim Y, Park S, Kim H, et al. Atg7-dependent canonical autophagy regulates the degradation of aquaporin 2 in prolonged hypokalemia. Sci Rep. 2019;9:3021 pubmed publisher
	Sonoda H, Oshikawa Hori S, Ikeda M. An Early Decrease in Release of Aquaporin-2 in Urinary Extracellular Vesicles After Cisplatin Treatment in Rats. Cells. 2019;8: pubmed publisher
	Kong M, Bak S, Han K, Kim J, Park J, Park K. Fragmentation of kidney epithelial cell primary cilia occurs by cisplatin and these cilia fragments are excreted into the urine. Redox Biol. 2019;20:38-45 pubmed publisher
	Otterpohl K, Hart R, Evans C, Surendran K, Chandrasekar I. Nonmuscle myosin 2 proteins encoded by Myh9, Myh10, and Myh14 are uniquely distributed in the tubular segments of murine kidney. Physiol Rep. 2017;5: pubmed publisher
	Li W, Jin W, Tsuji K, Chen Y, Nomura N, Su L, et al. Ezrin directly interacts with AQP2 and promotes its endocytosis. J Cell Sci. 2017;130:2914-2925 pubmed publisher
	Al Bataineh M, Li H, Ohmi K, Gong F, Marciszyn A, Naveed S, et al. Activation of the metabolic sensor AMP-activated protein kinase inhibits aquaporin-2 function in kidney principal cells. Am J Physiol Renal Physiol. 2016;311:F890-F900 pubmed publisher
	Mamenko M, Dhande I, Tomilin V, Zaika O, Boukelmoune N, Zhu Y, et al. Defective Store-Operated Calcium Entry Causes Partial Nephrogenic Diabetes Insipidus. J Am Soc Nephrol. 2016;27:2035-48 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-Aquaporin 2 Antibody (#AQP-002), (1:200).2. Anti-Aquaporin 2 Antibody, preincubated with a blocking peptide.

image 2 :

Expression of AQP2 in rat kidney - Immunohistochemical staining of rat kidney paraffin embedded section showing the inner medulla using Anti-Aquaporin 2 Antibody (#AQP-002), (1:100). Intense stain (brown color) is present in collecting ducts but not in thin segments of the loop of Henle. Hematoxilin is used as the counterstain.

product information

CAT :

AQP-002

SKU :

AQP-002-CF_0.2 ml

Product Name :

Anti-Aquaporin 2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P34080

Applications :

IC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP002

Homology :

Mouse - identical; human, sheep - 17/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2

Product Page - Scientific background :

Aquaporin 2 (AQP2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP0 to AQP12). Little is known about the function of the two newest members, AQP11 and AQP12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP2 together with AQP1, AQP4 and AQP5 belongs to the first group.1The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1AQP2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine.2Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressin receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP2. This phosphorylation causes the translocation of AQP2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.1,3,4

Applications may also work in :

IC IHC WB

Supplier :

Alomone Labs

Target :

AQP2, AQP-CD, WCH-CD

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 2 Channel

Long Description :

Anti-Aquaporin 2 Antibody (#AQP-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize the AQP2 channel from rat, mouse, and human samples.

Negative Control :

BLP-QP002

Positive Control :

Synonyms :

AQP2, AQP-CD, WCH-CD

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage