Anti-Aquaporin 2-ATTO Fluor-550 Antibody | Alomone Labs AQP-002-AO product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 2-ATTO Fluor-550 Antibody

catalog :

AQP-002-AO

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Hasegawa K, Yamaguchi Y, Tanaka M. Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise. Physiol Rep. 2021;9:e14670 pubmed publisher
Lee J, Kim S, Jo C, Lee C, Kim G. Urinary Concentration Defect and Renal Glycosuria in Cyclosporine-treated Rats. Electrolyte Blood Press. 2020;18:1-9 pubmed publisher
Kim C, Joo S, Kim I, Choi H, Bae E, Kim S, et al. Anti-Apoptotic Effect of G-Protein-Coupled Receptor 40 Activation on Tumor Necrosis Factor-α-Induced Injury of Rat Proximal Tubular Cells. Int J Mol Sci. 2019;20: pubmed publisher
Cheung P, Terlouw A, Janssen S, Brown D, Bouley R. Inhibition of non-receptor tyrosine kinase Src induces phosphoserine 256-independent aquaporin-2 membrane accumulation. J Physiol. 2019;597:1627-1642 pubmed publisher
Cherezova A, Tomilin V, Buncha V, Zaika O, Ortiz P, Mei F, et al. Urinary concentrating defect in mice lacking Epac1 or Epac2. FASEB J. 2019;33:2156-2170 pubmed publisher
Li W, Jin W, Tsuji K, Chen Y, Nomura N, Su L, et al. Ezrin directly interacts with AQP2 and promotes its endocytosis. J Cell Sci. 2017;130:2914-2925 pubmed publisher
Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
Schwaderer A, Wang H, Kim S, Kline J, Liang D, Brophy P, et al. Polymorphisms in Î±-Defensin-Encoding DEFA1A3 Associate with Urinary Tract Infection Risk in Children with Vesicoureteral Reflux. J Am Soc Nephrol. 2016;27:3175-3186 pubmed
Mamenko M, Dhande I, Tomilin V, Zaika O, Boukelmoune N, Zhu Y, et al. Defective Store-Operated Calcium Entry Causes Partial Nephrogenic Diabetes Insipidus. J Am Soc Nephrol. 2016;27:2035-48 pubmed publisher

image

image 1 :

Expression of Aquaporin 2 in rat kidney - Immunohistochemical staining of rat kidney paraffin embedded region using Anti-Aquaporin 2-ATTO Fluor-550 Antibody (#AQP-002-AO), (1:50), (Red). AQP2 is detected in collecting ducts but not in thin segments of the loop of Henle. Nuclei are visualized with Hoechst 33342 (blue).

image 2 :

Alomone Labs ?-Bungarotoxin-FITC inhibits ?7 nAChR heterologously expressed inXenopusoocytes. - A. Time course of?-Bungarotoxin-FITC(#B-100-F) action on ?7 nAChR currents elicited every 50 sec by transient 2 sec applications of 100 M ACh + 0.3 ?M PNU-120596 while membrane potential was held at -80 mV. Application of 0.5 M?-Bungarotoxin-FITCsignificantly inhibits the currents (green). B. Superimposed traces of ?7 nAChR currents in the absence (black) and presence (green) of 0.5 M ?-Bungarotoxin-FITC (taken from the experiment in A).

product information

CAT :

AQP-002-AO

SKU :

AQP-002-AO_50 mcl

Product Name :

Anti-Aquaporin 2-ATTO Fluor-550 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P34080

Applications :

IC IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP002

Homology :

Mouse - identical; human, sheep - 17/18 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2

Product Page - Scientific background :

Aquaporin 2 (AQP2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP0 to AQP12). Little is known about the function of the two newest members, AQP11 and AQP12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP3, AQP7, AQP9 and AQP10.1The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1AQP2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine.2Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressin receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP2. This phosphorylation causes the translocation of AQP2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.1,3,4

Applications may also work in :

IC IF IHC

Supplier :

Alomone Labs

Target :

AQP2, AQP-CD, WCH-CD

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 2 Conjugated to the Fluorescent Dye ATTO-550

Long Description :

Anti-Aquaporin 2 Antibody (#AQP-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize the AQP2 channel from rat, mouse, and human samples. Anti-Aquaporin 2-ATTO Fluor-550 Antibody (#AQP-002-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-Aquaporin 2-ATTO Fluor-550 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AO

Positive Control :

Synonyms :

AQP2, AQP-CD, WCH-CD

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AQP-002), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage