Anti-Aquaporin 1 Antibody | Alomone Labs AQP-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Aquaporin 1 Antibody

catalog :

AQP-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig 6e	Miwa T, Wei F, Tomizawa K. Cdk5 regulatory subunit-associated protein 1 knockout mice show hearing loss phenotypically similar to age-related hearing loss. Mol Brain. 2021;14:82 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 4a, 5a immunohistochemistry; mouse; 1:400; loading ...; fig 1a, 2h, 2k western blot; mouse; 1:400; loading ...; fig 1l immunohistochemistry - paraffin section; rat; 1:400; loading ...; fig 2m	Li Q, Aalling N, Förstera B, Erturk A, Nedergaard M, Møllgård K, et al. Aquaporin 1 and the Na+/K+/2Cl- cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system. Fluids Barriers CNS. 2020;17:15 pubmed publisher
	Weber J, Rajan S, Schremmer C, Chao Y, Krasteva Christ G, Kannler M, et al. TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema. JCI Insight. 2020;5: pubmed publisher
	Laszczyk A, Higashi A, Patel S, Johnson C, Soofi A, Abraham S, et al. Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney. J Am Soc Nephrol. 2020;31:1212-1225 pubmed publisher
	Chung S, Kim S, Son M, Kim M, Koh E, Shin S, et al. Empagliflozin Contributes to Polyuria via Regulation of Sodium Transporters and Water Channels in Diabetic Rat Kidneys. Front Physiol. 2019;10:271 pubmed publisher
	Kong M, Bak S, Han K, Kim J, Park J, Park K. Fragmentation of kidney epithelial cell primary cilia occurs by cisplatin and these cilia fragments are excreted into the urine. Redox Biol. 2019;20:38-45 pubmed publisher
	Agbani E, Williams C, Li Y, van den Bosch M, Moore S, Mauroux A, et al. Aquaporin-1 regulates platelet procoagulant membrane dynamics and in vivo thrombosis. JCI Insight. 2018;3: pubmed publisher
	Zeming K, Salafi T, Shikha S, Zhang Y. Fluorescent label-free quantitative detection of nano-sized bioparticles using a pillar array. Nat Commun. 2018;9:1254 pubmed publisher
	Comerma Steffensen S, Carvacho I, Hedegaard E, Simonsen U. Small and Intermediate Calcium-Activated Potassium Channel Openers Improve Rat Endothelial and Erectile Function. Front Pharmacol. 2017;8:660 pubmed publisher
	Han S, Jang H, Noh M, Kim J, Kong M, Kim J, et al. Mitochondrial NADP+-Dependent Isocitrate Dehydrogenase Deficiency Exacerbates Mitochondrial and Cell Damage after Kidney Ischemia-Reperfusion Injury. J Am Soc Nephrol. 2017;28:1200-1215 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-Aquaporin 1 Antibody (AQP-001), (1:400).2. Anti-Aquaporin 1 Antibody, preincubated with Aquaporin 1 Blocking Peptide (#BLP-QP001).

image 2 :

Expression of AQP1 in rat kidney - Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1 Antibody (#AQP-001), (1:100). AQP1 staining (brown) is present in both distal (DT) and proximal (PT) convoluted tubules in the renal cortex. Note that a weak staining is also present in collecting ducts (CD) while glomeruli (Gl) are negative. Hematoxilin is used as the counterstain.

image 3 :

Expression of Aquaporin 1 in mouse kidney - Immunohistochemical staining of mouse kidney sections using Anti-Aquaporin 1Antibody (#AQP-001). AQP1 staining (red) along the thin limb of Henle cells does not change following ischemia.Adapted from Kim J.I.et al.(2013)Am. J. Physiol.304F1283. with permission of The American Physiological Society.

product information

CAT :

AQP-001

SKU :

AQP-001-CF_0.2 ml

Product Name :

Anti-Aquaporin 1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P29972

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-QP001

Homology :

Rat, mouse, cattle, sheep - identical; dog - 18/19 amino acid residues identical; frog (Rana esculenta), toad (Bufo marinus) - 14/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1

Product Page - Scientific background :

Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2, AQP-4 and AQP-5 belongs to the first group1.Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration2. In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Short Description :

A Rabbit Polyclonal Antibody to Aquaporin 1 Channel

Long Description :

Anti-Aquaporin 1 Antibody (#AQP-001) is a highly specific antibody directed against an epitope of the human AQP1 channel. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP1 from rat, mouse, and human samples.

Negative Control :

BLP-QP001

Positive Control :

Synonyms :

AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AQP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC Whole Tissue Staining

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage