Anti-IQGAP1 Antibody | Alomone Labs APZ-055 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-IQGAP1 Antibody

catalog :

APZ-055

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1, 2. Anti-IQGAP1 Antibody (#APZ-055), (1:200). 3, 4. Anti-IQGAP1 Antibody, preincubated with IQGAP1 Blocking Peptide (#BLP-PZ055) (#BLP-PZ055).

image 2 :

Expression of IQGAP1 in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IQGAP1 Antibody (#APZ-055), (1:200), followed by goat anti-rabbit-AlexaFluor-488. IQGAP1 immunoreactivity appears in Purkinje neurons (horizontal arrows) and their dendrites (vertical arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APZ-055

SKU :

APZ-055-CF_0.2 ml

Product Name :

Anti-IQGAP1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P46940

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ055

Homology :

Mouse, rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KAYQDRLAYLRSHKDE, corresponding to amino acid residues 793 - 808 of human IQGAP1

Product Page - Scientific background :

IQGAP1 is a 190 kDa (1657 amino acid) protein that is ubiquitously expressed and contains six distinct protein-interacting domains with six coiled coil regions1.The Ras GTPase-activating-like protein is a scaffold protein involved in regulating various cellular processes; from organizing the actin cytoskeleton, transcription, cell adhesion, and regulating the cell cycle2,3.IQGAP1 binds to activated CDC42 but does not stimulate its GTPase activity and it may have a role in cell cycle progression following DNA damage1,2. The protein also regulates NR2A NMDA receptor signaling where it influences cognitive processes4. It also regulates MAPK and Wnt/β-catenin signaling pathways5. It is thought to contribute to tumorigenesis by stimulating cell motility and invasion6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195

Short Description :

A Rabbit Polyclonal Antibody to IQGAP1

Long Description :

Anti-IQGAP1 Antibody (#APZ-055) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IQGAP1 from rat, mouse, and human samples.

Negative Control :

BLP-PZ055

Positive Control :

Synonyms :

IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

IQGAP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage