Anti-GIPC1 Antibody | Alomone Labs APZ-045 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GIPC1 Antibody

catalog :

APZ-045

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1,2. Anti-GIPC1 Antibody (#APZ-045), (1:400).3,4. Anti-GIPC1 Antibody, preincubated with GIPC1 Blocking Peptide (#BLP-PZ045).

image 2 :

Expression of GIPC1 in mouse and rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-GIPC1 Antibody (#APZ-045), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. In mouse brain GIPC1 staining (green) appears in neurons of the pyramidal layer (P) and their apical dendrites (arrows). B. In rat brain similar staining is observed. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APZ-045

SKU :

APZ-045-CF_0.2 ml

Product Name :

Anti-GIPC1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O14908

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ045

Homology :

Rat, mouse - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KAPPLVENEEAEPGR, corresponding to amino acid residues 10-24 of human GIPC1

Product Page - Scientific background :

GIPC1 belongs to a family of evolutionarily conserved adaptor proteins responsible for the trafficking of numerous transmembrane proteins via endocytosis. Members of GIPC (GAIP interacting protein, C-terminus) have three main domains responsible for protein interactions; a PDZ domain which is flanked by GH1 and GH2 domains. GH1 domain is responsible for the dimerization of the protein, while GH2 is important for the interaction of GIPC1 with MYO6, a retrograde motor protein involved in protein trafficking. The PDZ domain of GIPC1 is responsible for the protein to interact with numerous proteins; namely β1-adrenoceptor, D2 dopamine receptor, GLUT1, TrkA receptor just to name a few. The zebrafish ortholog also interacts with Neuropilin-21,2.GIPC1 expression is upregulated in breast, ovarian and pancreas cancers2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein, RGS-GAIP-interacting protein, Synectin, NIP, TIP-2

Short Description :

A Rabbit Polyclonal Antibody to GIPC1

Long Description :

Anti-GIPC1 Antibody (#APZ-045) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GIPC1 from human, rat, and mouse samples.

Negative Control :

BLP-PZ045

Positive Control :

Synonyms :

PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein, RGS-GAIP-interacting protein, Synectin, NIP, TIP-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GIPC1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage