Anti-DLGAP1/GKAP Antibody | Alomone Labs APZ-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-DLGAP1/GKAP Antibody

catalog :

APZ-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200).3,4. Anti-DLGAP1/GKAP Antibody, preincubated with DLGAP1/GKAP Blocking Peptide (#BLP-PZ041).

image 2 :

Expression of GKAP in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GKAP staining (green) in hippocampal CA3 region is detected in neurons (arrows) of CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APZ-041

SKU :

APZ-041-CF_0.2 ml

Product Name :

Anti-DLGAP1/GKAP Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97836

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ041

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KISNEHSPKLQIRSH, corresponding to amino acid residues 397-411 of rat DLGAP1

Product Page - Scientific background :

DLGAP1/GKAP (discs large homolog associated protein 1/guanylate kinase associated protein) is a member of the DLGAP family, scaffold proteins localized to the postsynaptic density. The protein is involved in signaling to and from glutamate receptors, it serves as a key adaptor protein of NMDA receptors, involved in learning and memory and synaptic plasticity in the central nervous system1,2.DLGAP family comprises five conserved proteins, DLGAP1-DLGAP5. DLGAP1-DLGAP4 proteins interact directly with both DLG and SHANK through their multiple domains. DLGAP1-4 proteins are implicated in several neurological disorders including schizophrenia, autism, obsessive compulsive disorder and cerebellar ataxia1.DLGAP1 is highly expressed in the brain; Dlgap1 mRNA is detected in the cortex, hippocampus, olfactory bulb, striatum and thalamus1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1

Short Description :

A Rabbit Polyclonal Antibody to DLGAP1/GKAP/SAPAP1

Long Description :

Anti-DLGAP1/GKAP Antibody (#APZ-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DLGAP1 from human, mouse, and rat samples.

Negative Control :

BLP-PZ041

Positive Control :

Synonyms :

DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DLGAP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage