Anti-Gamma-Synuclein Antibody | Alomone Labs APZ-037 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Gamma-Synuclein Antibody

catalog :

APZ-037

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1, 2. Anti-Gamma-Synuclein Antibody (#APZ-037), (1:200). 3, 4. Anti-Gamma-Synuclein Antibody, preincubated with Gamma-Synuclein Blocking Peptide (#BLP-PZ037).

image 2 :

Expression of Gamma-Synuclein in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Gamma-Synuclein Antibody (#APZ-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Gamma-Synuclein immunoreactivity (green) appears in blood vessels (arrows). B. Pre-incubation of the antibody with Gamma-Synuclein Blocking Peptide (#BLP-PZ037), suppresses staining. Cell nuclei are stained with DAPI (blue). G = granule layer.

product information

CAT :

APZ-037

SKU :

APZ-037-CF_0.2 ml

Product Name :

Anti-Gamma-Synuclein Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O76070

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ037

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RKEDLEPPAQDQE, corresponding to amino acid residues 96 - 108 of rat γ-Synuclein

Product Page - Scientific background :

γ-synuclein is a 127 amino acids protein that belongs to the synuclein family of proteins. Synucleins are natively unfolded proteins, i.e. they have no secondary or tertiary structure in solution and hence these proteins are heat stable. The unusual biophysical properties of synucleins are rooted in their characteristic primary sequence that includes a highly conserved N-terminal domain with an 11- residue repeat and a less conserved acidic C-terminal domain that contains many proline residues1.Mammalian γ-synuclein, encoded by BCSG1 (breast cancer specific gene 1), was discovered in a screen for breast cancer markers2. It is expressed in the peripheral nervous system in primary sensory neurons, sympathetic neurons, and motor neurons. It is also detected in the brain3, olfactory epithelium and breast/ovarian tumors4. Indeed, exogenous expression of this protein increases the invasive and metastatic potential of breast tumors5.The roles of synucleins are not fully elucidated but this group of conserved and unusual proteins contributes to diverse disease processes spanning neurodegenerative disease and cancer and hence, studying the synucleins specific cellular activity is very tempting and promising.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

γ-Synuclein, Breast Cancer-Specific Gene 1 Protein, BCSG1, Persyn, Synoretin, SNCG

Short Description :

A Rabbit Polyclonal Antibody to Gamma-Synuclein

Long Description :

Anti-Gamma-Synuclein Antibody (#APZ-037) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot analysis. It has been designed to recognize γ-Synuclein from mouse and rat samples. The antibody will not recognize the protein from human samples.

Negative Control :

BLP-PZ037

Positive Control :

Synonyms :

γ-Synuclein, Breast Cancer-Specific Gene 1 Protein, BCSG1, Persyn, Synoretin, SNCG

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SNCG

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage