Anti-AKAP12 Antibody | Alomone Labs APZ-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-AKAP12 Antibody

catalog :

APZ-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Hu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, et al. Flotillin-1 Interacts With and Sustains the Surface Levels of TRPV2 Channel. Front Cell Dev Biol. 2021;9:634160 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Anti-AKAP12 Antibody (#APZ-033), (1:200).3,4. Anti-AKAP12 Antibody, preincubated with AKAP12 Blocking Peptide (#BLP-PZ033).

image 2 :

Expression of AKAP12 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-AKAP12 Antibody (#APZ-033), (1:400), followed by goat anti-rabbit-AlexaFluor-488. AKAP12 immunoreactivity (green) is detected in Purkinje cells and their dendritic trees (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APZ-033

SKU :

APZ-033-CF_0.2 ml

Product Name :

Anti-AKAP12 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WTQ5

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ033

Homology :

Rat - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GNLTKESPDTNGPK, corresponding to amino acid residues 1639-1652 of mouse AKAP12

Product Page - Scientific background :

The A-kinase anchoring protein 12 (AKAP12) belongs to a family of scaffold proteins, which anchor signaling proteins such as PKA in a spatiotemporal manner4. AKAPs generally bind PKA at their N-terminus, while the sequence at their C-terminus determines their spatial location4. To date, more than 50 AKAPs have been identified and perceived to integrate signaling pathways by anchoring multi-protein complexes1.AKAP12 integrates diverse signaling pathways such as PKA, PKC, and β2-adrenergic receptor7,8. Similar to other members of this protein-family, AKAP12 contains a PKA binding domain. In addition, AKAP12 also contains a docking site for PKC2,6 and a myristoyl acid that sequesters it to the plasma membrane7.AKAP12 is widely expressed in a variety of tissues with the highest expression in male and female reproductive organs, skeletal muscle, kidneys, and urinary tract5.One of the most characterized activities of AKAP12 is regulation of the cell cycle3. AKAP12 controls two fundamental cell processes such as the G1/S transition and cytokinesis. In that being said, studies in NIH-3T3 fibroblasts, showed that ectopic expression of AKAP12 reduced cyclin D1 levels and the phosphorylation of its downstream target Rb, and consequently attenuated cell-cycle progression3. Furthermore, in HeLa cells, knockdown of AKAP12 resulted in profound changes in cell morphology, which indicates the inability of cells to divide3.Given that AKAP12 plays a pivotal role in cell cycle regulation, it is not surprising that in some cases it is involved in cancer malignancies. It is now clear that AKAP12 is a key driver of gastrointestinal cancers such as colorectal cancer and hepatocellular carcinomas8.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS

Short Description :

A Rabbit Polyclonal Antibody to AKAP12

Long Description :

Anti-AKAP12 Antibody (#APZ-033) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Gravin from mouse, rat, and human samples.

Negative Control :

BLP-PZ033

Positive Control :

Synonyms :

A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AKAP12

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage