Anti-SynGAP Antibody | Alomone Labs APZ-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SynGAP Antibody

catalog :

APZ-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2 Anti-SynGAP Antibody (#APZ-032), (1:400).3,4. Anti-SynGAP Antibody, preincubated with SynGAP Blocking Peptide (#BLP-PZ032).

image 2 :

Expression of SynGAP in mouse globus pallidus and rat lateral septum - Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections using Anti-SynGAP Antibody (#APZ-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. SynGAP staining (green) in mouse brain is detected in neuropil (arrows) in the globus pallidus (GP) that is markedly stronger than in the striatum. B. SynGAP staining in rat brain sections shows lateral septum labeling of profiles of neuronal processes (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APZ-032

SKU :

APZ-032-CF_0.2 ml

Product Name :

Anti-SynGAP Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QUH6

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ032

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KSMDESRLDRVKEYE, corresponding to amino acid residues 1185-1199 of rat SynGAP

Product Page - Scientific background :

SynGAP1 is a major signaling protein that plays a pivotal role in regulating fundamental molecular changes in dendritic spine synaptic morphological and functional modifications.SynGAP1 functions as a downstream component of NMDA receptor-associated signaling and of AMPA receptor trafficking to excitatory postsynaptic membrane. SynGAP1 is mainly expressed in the hippocampus and cortex.SynGAP1 is a cytosolic protein without a signal peptide or any transmembrane domain. The N-terminal half of SYNGAP1 has a Ras-GAP domain, along with the region that is loosely homologous to Pleckstrin homology and a C2 domain which is potentially involved in binding Ca2+ and phospholipids. Interestingly, the alignment of GAP domain of SynGAP1 with other Ras-GAPs suggests that the amino acids in the GAP domain are crucial for interacting with Ras and for stimulating Ras-GTPase activity. The C-terminal half consists of a repeat of 10 histidines that may be involved in metal chelation, several potential serine and tyrosine phosphorylation sites and a T/SXV motif necessary for interaction with SAP102 and PSD-95.SynGAP1 has been implicated in the pathogenesis of intellectual disability. de novo out-of-frame deletions were identified in patients with intellectual disability presenting with microcephaly and generalized epilepsy while de novo microdeletions in chromosome region 6p21.3 were observed in patients with severe language impairment in addition to epilepsy. SynGAP1 may also be involved in the pathogenesis of schizophrenia through altered regulation of the NMDA receptor complex.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

SynGAP1, Synaptic Ras GTPase-activating protein 1, Synaptic Ras-GAP 1, Neuronal RasGAP, p135 SynGAP

Short Description :

A Rabbit Polyclonal Antibody to SynGAP

Long Description :

Anti-SynGAP Antibody (#APZ-032) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SynGAP from human, mouse, and rat samples.

Negative Control :

BLP-PZ032

Positive Control :

Synonyms :

SynGAP1, Synaptic Ras GTPase-activating protein 1, Synaptic Ras-GAP 1, Neuronal RasGAP, p135 SynGAP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SYNGAP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage