Anti-Homer3 Antibody | Alomone Labs APZ-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Homer3 Antibody

catalog :

APZ-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1,2. Anti-Homer3 Antibody (#APZ-028), (1:200).3,4. Anti-Homer3 Antibody, preincubated with Homer3 Blocking Peptide (#BLP-PZ028).

image 2 :

Expression of Homer3 in rat nucleus basalis - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Homer3 Antibody (#APZ-028), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. Homer3 immunoreactivity (green) appears in cells of the horizontal diagonal band (HDB), (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APZ-028

SKU :

APZ-028-CF_0.2 ml

Product Name :

Anti-Homer3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z2X5

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ028

Homology :

Human - identical; mouse - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RSQSADAPGPTERER, corresponding to amino acid residues 155 - 169 of rat Homer3

Product Page - Scientific background :

The HOMER protein family includes Homer1, 2, and 3. These proteins are mainly expressed in the neuronal post-synaptic density (PSD) where they play a role in post-synaptic signaling and receptor trafficking by forming multivalent links with various receptors and PSD scaffolding proteins. The expression level of Homer proteins in non-neural tissues is very low in comparison with that in the nervous system, HOMER3 also expressed in the lung, spleen, kidney and ovary. In the mouse brain HOMER3 is concentrated in the dendritic spines of Purkinje cells connecting the parallel fibers and is also present in their axons, In the hippocampus, HOMER3 is concentrated in the CA2-CA3 regions1,2.All three proteins in the HOMER family have several isoforms as a result of alternative splicing, HOMER3 are shown to exist in both long and short forms, the latter without the C-terminal coiled-coil domains. HOMER3 mRNA is subject to alternative splicing at five different sites giving rise to ten variants of HOMER3 proteins. HOMER1b and HOMER3 have been shown to form a tetramer via the carboxy-terminal domain3.HOMER3 directly binds to type I metabotropic glutamate receptors, which act via phospholipase C to stimulate IP3-mediated release of Ca2+ from intracellular vesicles3.HOMER proteins have also been implicated in axon guidance during brain development4.HOMER3 can regulate transcription and play a very important role in the differentiation and development for some tissues such as muscle and the nervous system. Studies show that HOMER3 abnormal expression plays a role in acute myeloid leukemia (AML). Heterologous expression of HOMER3 in K562 cells inhibits proliferation, influences the cell cycle profile, affects apoptosis and also promotes cell differentiation5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3

Short Description :

A Rabbit Polyclonal Antibody to Homer3

Long Description :

Anti-Homer3 Antibody (#APZ-028) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer3 from human, rat, and mouse samples.

Negative Control :

BLP-PZ028

Positive Control :

Synonyms :

Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HOMER3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage