Anti-Homer1 Antibody | Alomone Labs APZ-026 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Homer1 Antibody

catalog :

APZ-026

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain synaptosomal fractions (lanes 2 and 4): - 1,2. Anti-Homer1 Antibody (#APZ-026), (1:500).3,4. Anti-Homer1 Antibody, preincubated with Homer1 Blocking Peptide (#BLP-PZ026).

image 2 :

Expression of Homer1 in mouse hippocampus and striatum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Homer1 Antibody (#APZ-026), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A. Homer1 staining (green) in hippocampal dentate gyrus region, is detected in neuronal soma (arrows). B. Homer1 staining in the striatum region, also shows labeling in neuronal soma (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APZ-026

SKU :

APZ-026-CF_0.2 ml

Product Name :

Anti-Homer1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z214

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ026

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1

Product Page - Scientific background :

Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.The long form of Homer1 assembles group 1 metabotropic glutamate receptors mGluR1 and mGluR5 in large macromolecular complexes at synapses as well as with components of the NMDA receptor complex at the neuronal cell membrane. In contrast, the short form of Homer1 lacks the ability of linking mGluR1/5 to synaptic proteins and functions as an endogenous negative modulator of the mGluR1/5-inositol 1,4,5-trisphosphate receptor signaling complex. Transgenic mice with overexpressed Homer1 in striatal medium spiny neurons demonstrate normal development of striatal structure and afferent/efferent connectivity. However, motor performance in behavioral tasks and striatal responses to the psychomotor stimulant amphetamine are significantly altered in these mice2.At concentrations

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47

Short Description :

A Rabbit Polyclonal Antibody to Homer1

Long Description :

Anti-Homer1 Antibody (#APZ-026) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer1 from human, mouse, and rat samples. The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Negative Control :

BLP-PZ026

Positive Control :

Synonyms :

Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HOMER1

AB Specifictiy :

The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage