Anti-α1-Syntrophin (SNTA1) Antibody | Alomone Labs APZ-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-α1-Syntrophin (SNTA1) Antibody

catalog :

APZ-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat skeletal muscle membranes (lanes 2 and 4): - 1,2. Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:400).3,4. Anti-α1-Syntrophin (SNTA1) Antibody, preincubated with α1-Syntrophin/SNTA1 Blocking Peptide (#BLP-PZ021).

image 2 :

Expression of Syntrophin alpha 1 in mouse neocortex - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:400), followed by anti-rabbit-AlexaFluor-488 antibody (green). Syntrophin alpha 1 staining (green) appears in blood vessels (horizontal arrows) and in the pia mater (vertical arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

APZ-021

SKU :

APZ-021-CF_0.2 ml

Product Name :

Anti-α1-Syntrophin (SNTA1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61234

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ021

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, PH1 domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RQPSSPGPQPRNLSE, corresponding to amino acid residues 191-205 of mouse α1-syntrophin

Product Page - Scientific background :

α1-Syntrophin is a component of the dystrophin scaffold-protein complex that acts in recruiting proteins through their multiple protein-protein interaction motifs to the cytoplasmic side of plasma membranes. Proteins interacting with α1-syntrophin include voltage-gated sodium channels, neuronal nitric oxide synthase, calmodulin, ankyrin repeat-rich membrane spanning protein, G-proteins and more. The syntrophin family comprises five members: α1-syntrophin, β1-syntrophin, β2-syntrophin, γ1-syntrophin, and γ2-syntrophin1.α1-Syntrophin is a peripheral cytoplasmic membrane protein in skeletal and cardiac muscle cells1.The α1-syntrophin structure contains four conserved domains, two pleckstrin homology domains (PH1 and PH2), a PSD95-disks large-ZO1 (PDZ) domain and a syntrophin unique (SU) COOH-terminal domain2. The pleckstrin domains are responsible for the recruitment of proteins to the sarcolemma. The PDZ domain is inserted within the PH1 domain and binds to NOS-1 in skeletal muscle. This domain also binds to the C-terminus of the Kir2.1 channel and also to NaV1.5 and so allowing them to interact. The SU domain binds syntrophin to dystrophin2,3. α1-Syntrophin is also responsible for the site-specific anchoring of AQP4 protein. α1-Syntrophin deletion leads to the disruption of the polarized subcellular expression of AQP4 in the perivascular membranes4. Several studies have shown that syntrophin-deficiency in Duchenne muscular dystrophy can ultimately lead to muscle degeneration. Syntrophin-deficiency was also shown to reduce levels of nNOS in mice5,6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1

Short Description :

A Rabbit Polyclonal Antibody to α1-Syntrophin (SNTA1)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse syntrophin alpha 1. Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021) can be used in western blot and immunohistochemistry applications. It has been designed to recognize syntrophin alpha 1 from mouse, rat and human samples.

Negative Control :

BLP-PZ021

Positive Control :

Synonyms :

Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SNTA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage