Anti-Cortactin Antibody | Alomone Labs APZ-018 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Cortactin Antibody

catalog :

APZ-018

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-Cortactin Antibody (#APZ-018), (1:1000). 3, 4. Anti-Cortactin Antibody, preincubated with Cortactin Blocking Peptide (#BLP-PZ018).

product information

CAT :

APZ-018

SKU :

APZ-018-CF_0.2 ml

Product Name :

Anti-Cortactin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q60598

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ018

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin

Product Page - Scientific background :

Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63-65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN

Short Description :

A Rabbit Polyclonal Antibody to Cortactin

Long Description :

Anti-Cortactin Antibody (#APZ-018) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cortactin from rat, mouse, and human samples.

Negative Control :

BLP-PZ018

Positive Control :

Synonyms :

Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CTTN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:1000

more info or order :

Alomone Labs product webpage