Anti-Shank3 Antibody | Alomone Labs APZ-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Shank3 Antibody

catalog :

APZ-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions: - 1,2. Anti-Shank3 Antibody (#APZ-013), (1:500).3,4. Anti-Shank3 Antibody, preincubated with Shank3 Blocking Peptide (#BLP-PZ013).

image 2 :

Expression of Shank3 in rat and mouse parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections using Anti-Shank3 Antibody (#APZ-013), (1:400), followed by anti-rabbit-Cy3 antibody (red). Shank3 staining of both rat (A) and mouse (B) brain sections, appears in neuron processes (horizontal arrows). Nuclei are stained with DAPI (blue).

image 3 :

Expression of Shank3 in rat and mouse parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sectionsusingAnti-Shank3Antibody (#APZ-013) (1:400) followed by anti-rabbit-Cy3 antibody (red). Shank3 staining of both rat (A) and mouse (B) brain sections appears in neuron processes (horizontal arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

APZ-013

SKU :

APZ-013-CF_0.2 ml

Product Name :

Anti-Shank3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JLU4

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ013

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, between the PDZ and the SAM domains.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EKLPGSLRKGIPRTK, corresponding to amino acid residues 841-855 of rat Shank3

Product Page - Scientific background :

Shank3/ProSAP2 is one of three members of the Shank/ProSAP family of proteins which contain five conserved protein domains - an ankyrin repeat (ANK), a Src homology 3 (SH3), a PSD-95/Discs large/ZO-1 (PDZ), a proline-rich region containing homer- and cortactin-binding sites (Pro), and a sterile alpha motif (SAM)1. Shank proteins localize in the postsynaptic density (PSD) of excitatory synapses where they function as master scaffolding proteins by interacting directly or indirectly with various proteins, including major types of glutamate receptors - NMDARs, AMPARs, and mGluRs - via different domains2. Shank3 plays important roles in the formation, maturation, and maintenance of synapses3.Shank3 is expressed abundantly in heart and moderately in brain (including the cortex, hippocampus and cerebellum) and spleen4. Human genetic studies strongly support the notion that molecular defects of Shank3 contribute to autism spectrum disorders (ASD) which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests5. In addition, Shank3 mutations were also reported in patients with childhood-onset schizophrenia and intellectual disability6.Furthermore, haplo insufficiency of Shank3 gene causes a developmental disorder, 22q13.3 deletion syndrome (known as Phelan-McDermid syndrome), that is characterized by severe expressive language and speech delay, hypotonia, global developmental delay, and autistic behavior3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, Proline-rich synapse-associated protein 2, ProSAP2

Short Description :

A Rabbit Polyclonal Antibody to Shank3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Shank3. Anti-Shank3 Antibody (#APZ-013) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Shank3 from rat, mouse and human samples.

Negative Control :

BLP-PZ013

Positive Control :

Synonyms :

SH3 and multiple ankyrin repeat domains protein 3, SPANK-2, Proline-rich synapse-associated protein 2, ProSAP2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SHANK3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage