Anti-Shank1 Antibody | Alomone Labs APZ-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Shank1 Antibody

catalog :

APZ-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: - 1,2. Anti-Shank1 Antibody (#APZ-011), (1:400).3,4. Anti-Shank1 Antibody, preincubated with Shank1 Blocking Peptide (#BLP-PZ011).

image 2 :

Expression of Shank1 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Shank1 Antibody (#APZ-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Shank1 staining (green) is detected in interneurons (vertical arrows) and in the pyramidal layer (P, horizontal arrows). Nuclei are stained with DAPI (blue).

image 3 :

Expression of Shank1 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Shank1Antibody (#APZ-011) (1:300) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Shank1 staining (green) is detected in interneurons (vertical arrows) and in the pyramidal layer (P horizontal arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

APZ-011

SKU :

APZ-011-CF_0.2 ml

Product Name :

Anti-Shank1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WV48

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ011

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, between the SH3 and PDZ domains.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RSQEGRQESRSDKAK, corresponding to amino acid residues 614-628 of rat Shank1

Product Page - Scientific background :

Shank family proteins (shank1, shank2 and shank3) are neuronal scaffold proteins that organize a cytoskeleton-associated signaling complex at the postsynaptic density (PSD) of nearly all excitatory glutamatergic synapses in the mammalian brain1.The Shank family, including Shank1, has five conserved protein domains: 1. ankyrin repeat domain (ANK), 2. Src homology 3 (SH3) domain, 3. PSD-95/Discs large/ZO-1 (PDZ) domain, 4. proline-rich region containing homer and cortactin-binding sites (Pro), 5. sterile α motif (SAM) domain2. All these domains can act as sites for specific protein-protein interactions, and the presence of these multiple domains within a single polypeptide is consistent with a function as a scaffolding protein. Shanks interact directly or indirectly with all major types of glutamate receptors: NMDA receptors, AMPA receptors, and mGluRs via different domains. Shank proteins also interact with several actin regulatory proteins and L-type Ca2+ (CaV1.3) channels in medium spiny neurons of the striatum3.Shank1 mRNA, being synthesized in dendritic spine, can locally control the spine morphology. The Shank1 gene is located on chromosome 19q13.33 and contains 23 exons and two alternative promoters leading to two distinct isoforms4. Shank1 is highly expressed in the cortex and hippocampus5.Recent studies indicate that Shank1 is a causative gene for idiopathic autism spectrum disorders (ASD)1. In addition it was found that Shank1 has a role in working memory deficits in schizophrenia6. Shank1 knock-out mice display increased anxiety and decreased vocal communication7.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon

Short Description :

A Rabbit Polyclonal Antibody to Shank1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Shank1. Anti-Shank1 Antibody (#APZ-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Shank1 from human, rat, and mouse samples.

Negative Control :

BLP-PZ011

Positive Control :

Synonyms :

SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SHANK1

AB Specifictiy :

This antibody will recognize all known rat isoforms of Shank1 with the exception of isoform 2 (see Q9WV48-2).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage