Anti-SAP-97/DLG1 Antibody | Alomone Labs APZ-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SAP-97/DLG1 Antibody

catalog :

APZ-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6): - 1-3. Anti-SAP-97/DLG1 Antibody (#APZ-010), (1:200).4-6. Anti-SAP-97/DLG1 Antibody, preincubated with SAP-97/DLG1 Blocking Peptide (#BLP-PZ010).

image 2 :

Peptide (C)EYRSKLSQTEDRQLR corresponding to amino acid residues 17-31 of human SAP-97 (AccessionQ12959). N-terminus L27 domain.

image 3 :

Western blot analysis of human SH-SY5Y neuroblastoma (lane 1 and 3) and human HT-29 colon adenocarcinoma (lanes 2 and 4)cell line lysate: - 12.Anti-DPP10 (extracellular) Antibody (#APC-147) (1:200).34. Anti-DPP10 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APZ-010

SKU :

APZ-010-CF_0.2 ml

Product Name :

Anti-SAP-97/DLG1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q12959

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ010

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminus, L27 domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EYRSKLSQTEDRQLR, corresponding to amino acid residues 17-31 of human SAP-97

Product Page - Scientific background :

SAP-97 belongs to the MAGUK (membrane associated guanylate kinase) homologs along with PSD-95, PSD-93 and SAP102.Like other members, SAP-97 has three PDZ domains, one SH3 domain and an inactive GUK domain. Alternative splicing of SAP-97 gives to a number of different isoforms thereby complicating the understanding of its roles1.In the post-synaptic cell, SAP-97 is the only MAGUK to directly interact with the GluA1 AMPA receptor2. SAP-97 also directly interacts with NMDA receptors through the PDZ domain3,4.SAP-97 was shown to interact with KV1.45 and with KV4.2 where it directs the channel to spines in hippocampal neurons6. It can also interact with the channel in the heart7.Interactions with Kir channels have also been observed8,9.Thus, SAP-97 serves as a regulator of neuronal excitability and synaptic function. Roles for the protein in schizophrenia, Alzheimer's and Parkinson's disease and epilepsy have been found1.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Synapse-associated protein 97, Discs large homolog 1

Short Description :

A Rabbit Polyclonal Antibody to SAP-97

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human SAP-97. Anti-SAP-97/DLG1 Antibody (#APZ-010) can be used in western blot analysis. It has been designed to recognize SAP-97 from rat, mouse and human samples.

Negative Control :

BLP-PZ010

Positive Control :

Synonyms :

Synapse-associated protein 97, Discs large homolog 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DLG1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage