Anti-PSD-95 Antibody | Alomone Labs APZ-009 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PSD-95 Antibody

catalog :

APZ-009

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 1	Bodaleo F, Montenegro Venegas C, HenrÃquez D, Court F, Gonzalez Billault C. Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology. Sci Rep. 2016;6:30069 pubmed publisher
	Risner M, Pasini S, Chamling X, McGrady N, Goldberg J, Zack D, et al. Intrinsic Morphologic and Physiologic Development of Human Derived Retinal Ganglion Cells In Vitro. Transl Vis Sci Technol. 2021;10:1 pubmed publisher
	Chhibber A, Zhao L. ER? and ApoE isoforms interact to regulate BDNF-5-HT2A signaling and synaptic function in the female brain. Alzheimers Res Ther. 2017;9:79 pubmed publisher
	Chhibber A, Woody S, Karim Rumi M, Soares M, Zhao L. Estrogen receptor ? deficiency impairs BDNF-5-HT2A signaling in the hippocampus of female brain: A possible mechanism for menopausal depression. Psychoneuroendocrinology. 2017;82:107-116 pubmed publisher
	Hsu P, Ma Y, Fang Y, Huang J, Gan Y, Chang P, et al. Cullin 7 mediates proteasomal and lysosomal degradations of rat Eag1 potassium channels. Sci Rep. 2017;7:40825 pubmed publisher

image

image 1 :

Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Anti-PSD-95 Antibody (#APZ-009), (1:200).3,4. Anti-PSD-95 Antibody, preincubated with PSD-95 Blocking Peptide (#BLP-PZ009).

image 2 :

Expression of PSD-95 in mouse parietal cortex - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PSD-95 Antibody (#APZ-009), (1:400), followed by anti-rabbit-Cy3 antibody (red). Staining appears mainly in the pyramidal cell body (arrows). Nuclei were stained with DAPI (blue).

image 3 :

Peptide (C)KDWGSSSGSQGRED corresponding to amino acid residues 505-518 of human PSD-95 (AccessionP78352). Intracellular between the SH3 and the GK-like domains.

product information

CAT :

APZ-009

SKU :

APZ-009-CF_0.2 ml

Product Name :

Anti-PSD-95 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P78352

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ009

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, between the SH3 and the GK-like domains.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95

Product Page - Scientific background :

The postsynaptic density (PSD) is a membrane-associated protein specialized in postsynaptic signal transduction and processing. There are four PSD family members classified according to their molecular weight, including, PSD-95, PSD-93, synaptic associated proteins 97 kDa and 102 kDa. PSD-95 interacts with both ionotropic and metabotropic glutamate receptors via protein-protein interactions and plays a role in their precise assembly and spatial organization as well as coupling of these receptors to downstream signaling events1.PSD-95 contains a SH3 domain, a GK domain and three PDZ domains. The PDZ domains 1 and 2 of PSD-95 are separated by only a few residues, creating a rigid bond that may restrain interdomain flexibility. These two PDZ domains can have distinct or overlapping target-binding proteins. In contrast, PDZ3 of PSD-95 is located at the carboxyl terminal end of the protein and has a set of interacting proteins that are distinct from PDZ domains 1 and 2. The N-palmitoylation of PSD-95 at Cys-3 and Cys-5 residues is essential for enhanced synaptic responses and the trafficking of PSD-95 to the plasma membrane2. With its numerous domains, PSD-95 is often referred to as a scaffold protein, which localizes and traffics various receptors, cell adhesion molecules, ion channels, kinases and phosphatases to the synaptic membrane. PSD-95 also interacts with a variety of adaptor and cytoskeleton proteins, molecular motors, as well as protein synthesis machinery3.PSD-95 is found at the postsynaptic membrane of excitatory synapses4.PSD-95 null mice have severe learning defects and exhibit both facilitation of long-term potentiation and disruption of long-term depression5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15

Short Description :

A Rabbit Polyclonal Antibody to PSD-95

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope human PSD-95. Anti-PSD-95 Antibody (#APZ-009) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSD-95 from mouse, rat and human samples.

Negative Control :

BLP-PZ009

Positive Control :

Synonyms :

Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DLG4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage