Guinea Pig Anti-PSD-95 Antibody | Alomone Labs APZ-009-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-PSD-95 Antibody

catalog :

APZ-009-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1-2. Guinea Pig Anti-PSD-95 Antibody (#APZ-009-GP), (1:200).3-4. Guinea Pig Anti-PSD-95 Antibody, preincubated with PSD-95 Blocking Peptide (BLP-PZ009).

product information

CAT :

APZ-009-GP

SKU :

APZ-009-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-PSD-95 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P78352

Applications :

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PZ009

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, between the SH3 and the GK-like domains.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95

Product Page - Scientific background :

The postsynaptic density (PSD) is a membrane-associated protein specialized in postsynaptic signal transduction and processing. There are four PSD family members classified according to their molecular weight, including, PSD-95, PSD-93, synaptic associated proteins 97 kDa and 102 kDa. PSD-95 interacts with both ionotropic and metabotropic glutamate receptors via protein-protein interactions and plays a role in their precise assembly and spatial organization as well as coupling of these receptors to downstream signaling events1.PSD-95 contains a SH3 domain, a GK domain and three PDZ domains. The PDZ domains 1 and 2 of PSD-95 are separated by only a few residues, creating a rigid bond that may restrain interdomain flexibility. These two PDZ domains can have distinct or overlapping target-binding proteins. In contrast, PDZ3 of PSD-95 is located at the carboxyl terminal end of the protein and has a set of interacting proteins that are distinct from PDZ domains 1 and 2. The N-palmitoylation of PSD-95 at Cys-3 and Cys-5 residues is essential for enhanced synaptic responses and the trafficking of PSD-95 to the plasma membrane2. With its numerous domains, PSD-95 is often referred to as a scaffold protein, which localizes and traffics various receptors, cell adhesion molecules, ion channels, kinases and phosphatases to the synaptic membrane. PSD-95 also interacts with a variety of adaptor and cytoskeleton proteins, molecular motors, as well as protein synthesis machinery3.PSD-95 is found at the postsynaptic membrane of excitatory synapses4.PSD-95 null mice have severe learning defects and exhibit both facilitation of long-term potentiation and disruption of long-term depression5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15, MRD62

Short Description :

A Guinea Pig Polyclonal Antibody to PSD-95

Long Description :

Guinea Pig Anti-PSD-95 Antibody (#APZ-009-GP, formerly AGP-132) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot applications. It has been designed to recognize PSD-95 from rat, mouse, and human samples. The antigen used to immunize guinea pigs is the same as Anti-PSD-95 Antibody (#APZ-009) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-PZ009

Positive Control :

Synonyms :

Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15, MRD62

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DLG4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage