Anti-NHERF-1/EBP50 Antibody | Alomone Labs APZ-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NHERF-1/EBP50 Antibody

catalog :

APZ-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Zhang Q, Gefter J, Sneddon W, Mamonova T, Friedman P. ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. iScience. 2021;24:102770 pubmed publisher
Liu D, Song A, Qi X, van Vliet P, Xiao J, Xiong F, et al. Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel. Nat Commun. 2021;12:2551 pubmed publisher
Zhang Q, Xiao K, Paredes J, Mamonova T, Sneddon W, Liu H, et al. Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport. J Biol Chem. 2019;294:4546-4571 pubmed publisher

image

image 1 :

Western blotting of rat kidney membranes: - 1. Anti-NHERF-1/EBP50 Antibody (APZ-006), (1:200). 2. Anti-NHERF-1/EBP50 Antibody, preincubated with NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006).

image 2 :

Western blotting of rat kidney membranes: - 1.Anti-NHERF-1/EBP50 Antibody(APZ-006)(1:200). 2. Anti-NHERF-1/EBP50 Antibody preincubated with the control peptide antigen.

product information

CAT :

APZ-006

SKU :

APZ-006-CF_0.2 ml

Product Name :

Anti-NHERF-1/EBP50 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JJ19

Applications :

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ006

Homology :

Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Between the PDZ2 and the ERM binding domains.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1

Product Page - Scientific background :

The Na+/H+ exchange regulatory factor (EBP-50 or NHERF-1) is a 55 kD cytoplasmic protein adaptor that recruits a wide variety of cellular proteins.Many of the interacting proteins do so through the two tandem PDZ domains (protein-binding domains conserved in the mammalian synaptic protein PSD-95/DlgA/ZO-1) and the C-terminal ERM (ezrin, radixin, moesin) binding region.NHERF-1 was first identified as an adaptor necessary for the function of the Na+/H+ exchanger isoform 3 (NHE3) in renal apical cells.1 Since then it has been identified in cells of epithelial origin in several tissues such as gastrointestinal and lung.NHERF-1 has been shown to interact with a growing number of proteins including ion channels (ROMK, CFTR, P2Y1, TRPC4 and TRPC6), growth factor receptors (PDGFR), phospholipase C isoforms (PLCb1, PLCb2, PLCb3), non-receptor protein tyrosine kinases (YAP65) and several cytoskeletal proteins that link membrane proteins to the underlying actin cytoskeleton.2Recently, it has been shown that NHERF-1 expression was elevated in breast tumors compared to the expression in adjacent normal tissue.3

Applications may also work in :

Supplier :

Alomone Labs

Target :

Na+/H+ exchange regulatory cofactor 1, Ezrin-radixin-moesin-binding phosphoprotein 50, SLC9A3R1

Short Description :

A Rabbit Polyclonal Antibody to NHERF-1/EBP50

Long Description :

Anti-NHERF-1/EBP50 Antibody (#APZ-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NHERF-1 from rat, mouse, and human samples.

Negative Control :

BLP-PZ006

Positive Control :

Synonyms :

Na+/H+ exchange regulatory cofactor 1, Ezrin-radixin-moesin-binding phosphoprotein 50, SLC9A3R1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NHERF1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage