Anti-Caspr2 Antibody | Alomone Labs APZ-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Caspr2 Antibody

catalog :

APZ-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Caspr2 Antibody (#APZ-005), (1:200).3,4. Anti-Caspr2 Antibody, preincubated with Caspr2 Blocking Peptide (#BLP-PZ005).

image 2 :

Expression of Caspr2 in rat cerebellum - Immunohistochemical staining of rat brain frozen sections using Anti-Caspr2 Antibody (#APZ-005), (1:200), (green). A. Caspr2 appears in pinceau structures (arrows) and diffusely in the molecular layer (MOL). Staining of the same section for parvalbumin (red) labels Purkinje cells and demonstrates the position of the pinceau under Purkinje cells. B. Pre-incubation of the antibody with the Caspr2 peptide antigen blocks the staining, demonstrating specificity.

image 3 :

Expression of Caspr2 in rat cerebellum - Immunohistochemical staining of rat brain frozen sections using Anti-Caspr2Antibody (#APZ-005) (1:200) (green). A. Caspr2 appears in pinceau structures (arrows) and diffusely in the molecular layer (MOL). Staining of the same section for parvalbumin (red) labels Purkinje cells and demonstrates the position of the pinceau under Purkinje cells. B. Pre-incubation of the antibody with the Caspr2 peptide antigen blocks the staining demonstrating specificity.

product information

CAT :

APZ-005

SKU :

APZ-005-CF_0.2 ml

Product Name :

Anti-Caspr2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UHC6

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PZ005

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DPNFTETIDESKKEWLI, corresponding to amino acid residues 1315-1331 of human Caspr2

Product Page - Scientific background :

Contactin-associated protein 2 (Caspr2) is a member of the neurexin superfamily, a group of transmembrane proteins that mediate cell-to-cell interactions in the nervous system1,2.Neurexins are adhesion molecules expressed mainly at presynaptic locations that form trans-synaptic cell-to-cell adhesion complexes via binding to their postsynaptic partners, the neuroligins2.Caspr2, like other neurexin proteins, is a type I membrane protein that contains epidermal growth factor repeats, laminin G domains, an F5/8 type C domain, and fibrinogen-like domains in its extracellular domain1,2.Caspr2 is localized at the juxtaparanodes of myelinated axons, a specialized region that mediates interactions between neurons and glia during nervous system development1.The juxtaparanodal region is highly enriched with heteromultimers of the K+ channels KV1.1, KV1.2, and their cytoplasmic KVβ2 subunit, which may help in axon conduction stabilization and the maintenance of the internodal resting potential1. Caspr2 is essential for the targeted localization of these channels in the juxtaparanodal regions. Indeed, targeted disruption of Caspr2 resulted in a marked reduction in the accumulation of K+ channels at the juxtaparanodes in both peripheral and central nervous system axons3.Moreover, mutations in the Caspr2 gene have been implicated in multiple neurodevelopmental disorders, including Tourette syndrome, schizophrenia, epilepsy, autism, attention-deficit hyperactivity disorder and mental retardation4-6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Contactin-associated protein-like 2, CNTNAP2

Short Description :

A Rabbit Polyclonal Antibody to Caspr2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Caspr2. Anti-Caspr2 Antibody (#APZ-005) can be used in western blot and immunohistochemistry analysis. It has been designed to recognize Caspr2 from rat, mouse and human samples.

Negative Control :

BLP-PZ005

Positive Control :

Synonyms :

Contactin-associated protein-like 2, CNTNAP2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNTNAP2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage