antibody

Anti-PepT2/SLC15A2 (extracellular) Antibody

APT-002

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

APT-002-CF_0.2 ml

Anti-PepT2/SLC15A2 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q9ES07

IFC IHC LCI WB

Human Rat Mouse

Rabbit

BLP-PT002

Rat - 13 out of 15 amino acid residues identical Human - 12 out of 15 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, 5th loop.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)KDYGVSEYRTVQRGK, corresponding to amino acid residues 541 - 555 of mouse SLC15A2

Members of the proton-coupled oligopeptide transporter (POT) family are integral membrane proteins that consist of 12 transmembrane helices, having both the amino- and carboxy-terminus localized intracellularly. Four members of the POT family, called peptide transporter 1 (PEPT1, encoded by SLC15A1), peptide transporter 2 (PEPT2, encoded by SLC15A2), peptide/histidine transporter 1 (PHT1, encoded by SLC15A4) and peptide/histidine transporter 2 (PHT2, encoded by SLC15A3), have been identified in humans and other mammals1,2. They transport a wide range of di- and tripeptides and peptide-like drugs via an inwardly-directed proton gradient. PHT1 and PHT2 are also able to transport the free amino acid histidine across biological membranes3. PEPT1 was characterized as a high-capacity, low-affinity transporter4 and is expressed especially in the small intestine, where it facilitates the luminal uptake of di- and tripeptides, as well as peptidomimetics and was also found in placenta, liver, kidney, and pancreas5. Additionally, PEPT1 is suggested to mediate intracellular transport, as it was found in nuclei and lysosomes6. In contrast to PEPT1, PEPT2 was characterized as a high-affinity, low-capacity transporter7 and found to be expressed especially in kidney, responsible for the renal reabsorption of filtered di- and tripeptides, as well as peptide-like agents8. In addition, this transporter is present in brain, here with a particular role in peptide uptake out of the cerebrospinal fluid into the choroid plexus9, lung10, skin11 and mammary gland12. PEPT2 had significant expression in the choroid plexus epithelial cells13. The choroid plexus acts as a barrier between the blood and the cerebrospinal fluid (CSF) which surrounds the brain and contains neuronal nutrients and waste. PEPT2 has been implicated in the clearance of peptides from the CSF, acting as an efflux pump14,15 and ensuring CSF homoeostasis16,17. The expression of PEPT2 in the choroid plexus and the lung highlight the versatility of the transporters and the necessity for a deeper understanding of the transporters to assess the factors which influence their activity. This is also highly relevant to medicine, as PEPT1 and PEPT2 have been implicated in the transport and retention of peptidomimetic drugs18.PEPT2 expression has been observed in the lung10,12,19-22. Lung epithelial tissue was shown to contain PEPT2 messenger ribonucleic acid (mRNA), and white lung preparations are able to transport fluorophore conjugated peptide, indicating functional PEPT2 expression. It is not clear what the primary function of PEPT2 is in the lung. It is a possibility that PEPT2 functions as part of the innate immune system23.

IFC IHC LCI WB

Alomone Labs

Peptide Transporter 2, Solute Carrier Family 15 Member 2, Oligopeptide Transporter - Kidney Isoform, Kidney H+/Peptide Cotransporter

A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)

Anti-PepT2/SLC15A2 (extracellular) Antibody (#APT-002) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PepT2 from mouse, rat and human samples.

BLP-PT002

NA

Peptide Transporter 2, Solute Carrier Family 15 Member 2, Oligopeptide Transporter - Kidney Isoform, Kidney H+/Peptide Cotransporter

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC15A2

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:100

1:200-1:500