Anti-P2Y10 (extracellular) Antibody | Alomone Labs APR-110 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y10 (extracellular) Antibody

catalog :

APR-110

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-P2Y10 (extracellular) Antibody (#APR-110), (1:400). 3-4. Anti-P2Y10 (extracellular) Antibody, preincubated with P2Y10 (extracellular) Blocking Peptide (BLP-PR110).

image 2 :

Expression of P2Y10 in mouse striatum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-P2Y10 (extracellular) Antibody (#APR-110), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. P2Y10 immunoreactivity (green) appears in striatal cells (arrows). B. Pre-incubation of the antibody with P2Y10 (extracellular) Blocking Peptide (BLP-PR110), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APR-110

SKU :

APR-110-CF_0.2 ml

Product Name :

Anti-P2Y10 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BFU7

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR110

Homology :

Rat - 14 out of 16 amino acid residues identical Not recommended for human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NSTDS(S)FADLGYKQMD, corresponding to amino acid residues 165 -180 of mouse P2Y10

Product Page - Scientific background :

P2Y10 belongs to the family of G-protein coupled receptors (GPCRs) and was first described as a lymphoid-restricted receptor. Based on sequence homology, it was thought to be a member of the purinergic P2Y GPCR family and a nucleotide receptor 1. Later studies suggested that it was a receptor for lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P)2, although the findings were not confirmed by others.More recent studies using a TGFα shedding assay, showed P2Y10 as a receptor for lysophosphatidylserine (LysoPS), a bioactive lipid present in the central nervous system (CNS) and immune system 3,4. Nevertheless, P2Y10 is still classified by the International Union of Basic and Clinical Pharmacology, as an orphan class A GPCR receptor.P2Y10 putative ligand, LysoPS, has been found to activate at least two other class A orphan GPCRs named GPR34 and GPR174, that have also been named as LYPSR1 and LYPSR3 (respectively), with P2Y10 also named as LYPSR24.The biological function of P2Y10 has not been completely elucidated, but different studies point to a role in eosinophil degranulation5, involvement in neuroinflammation6 and regulation of T-cell migration7.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

P2Y Receptor Family Member 10, Putative P2Y Purinoceptor 10, P2Y-Like Receptor, LYPSR2, P2RY10

Short Description :

A Rabbit Polyclonal antibody to P2Y10 (extracellular)

Long Description :

Anti-P2Y10 (extracellular) Antibody (#APR-110) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize P2Y10 from mouse and rat samples. The antibody is unlikely to recognize human P2Y10.

Negative Control :

BLP-PR110

Positive Control :

Synonyms :

P2Y Receptor Family Member 10, Putative P2Y Purinoceptor 10, P2Y-Like Receptor, LYPSR2, P2RY10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY10

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage