Anti-P2Y13 Receptor (extracellular)-FITC Antibody | Alomone Labs APR-103-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y13 Receptor (extracellular)-FITC Antibody

catalog :

APR-103-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of P2Y13 by direct flow cytometry in live intact mouse BV-2 microglia cells: Cell surface detection of P2Y13 by direct flow cytometry in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-P2Y13 Receptor (extracellular)-FITC Antibody (#APR-103-F), (5µg) .

product information

CAT :

APR-103-F

SKU :

APR-103-F_15 mcl

Product Name :

Anti-P2Y13 Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9D8I2

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR103

Homology :

Mouse - 15 out of 16 amino acid residues identical Human, rat – 14 out of 16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, 3rd loop.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NKTD(S)RLENQLFIAKE, corresponding to amino acid residues 266 - 281 of mouse P2Y13

Product Page - Scientific background :

The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions. A new member of this family was recently identified, the P2Y13 receptor.1,2 The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12 receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gi class of G-proteins.1,2 The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung, liver and other peripheral organs.1,2 Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.3 P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.4

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94, SP174

Short Description :

A Rabbit Polyclonal Antibody to P2Y13 Receptor (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize P2Y13 from mouse, rat and human samples. Anti-P2Y13 Receptor (extracellular)-FITC Antibody (#APR-103-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94, SP174

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY13

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-103), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage