Anti-P2Y2 Receptor (extracellular) Antibody | Alomone Labs APR-102 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y2 Receptor (extracellular) Antibody

catalog :

APR-102

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Nardin C, Peres C, Putti S, Orsini T, Colussi C, Mazzarda F, et al. Connexin Hemichannel Activation by S-Nitrosoglutathione Synergizes Strongly with Photodynamic Therapy Potentiating Anti-Tumor Bystander Killing. Cancers (Basel). 2021;13: pubmed publisher
Sophocleous R, Miles N, Ooi L, Sluyter R. P2Y2 and P2X4 Receptors Mediate Ca2+ Mobilization in DH82 Canine Macrophage Cells. Int J Mol Sci. 2020;21: pubmed publisher
Jiang P, Xing F, Guo B, Yang J, Li Z, Wei W, et al. Nucleotide transmitters ATP and ADP mediate intercellular calcium wave communication via P2Y12/13 receptors among BV-2 microglia. PLoS ONE. 2017;12:e0183114 pubmed publisher

image

image 1 :

Western blot analysis of mouse heart lysate (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat brain lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8): - 1-4. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200).5-8. Anti-P2Y2 Receptor (extracellular) Antibody, preincubated with P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102).

image 2 :

Expression of P2Y2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. P2RY2 immunoreactivity (green) in the hippocampal dentate gyrus appears in blood vessels (horizontal arrows) and in neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APR-102

SKU :

APR-102-CF_0.2 ml

Product Name :

Anti-P2Y2 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41232

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR102

Homology :

Rat, mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2

Product Page - Scientific background :

P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation1.The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y142-4.The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway5. It has become a leading target for the treatment of cystic fibrosis. Activation of P2RY2 increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 2, P2RY2, P2U1

Short Description :

A Rabbit Polyclonal Antibody to P2Y2 Receptor

Long Description :

Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, live cell imaging, and immunohistochemistry applications. It has been designed to recognize P2RY2 from rat, mouse, and human samples.

Negative Control :

BLP-PR102

Positive Control :

Synonyms :

P2Y purinoceptor 2, P2RY2, P2U1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage