Anti-P2Y2 Receptor (extracellular)-FITC Antibody | Alomone Labs APR-102-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y2 Receptor (extracellular)-FITC Antibody

catalog :

APR-102-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Hevia M, Castro P, Pinto K, Reyna Jeldes M, Rodr xed guez Tirado F, Robles Planells C, et al. Differential Effects of Purinergic Signaling in Gastric Cancer-Derived Cells Through P2Y and P2X Receptors. Front Pharmacol. 2019;10:612 pubmed publisher

image

image 1 :

Cell surface detection of P2RY2 in live intact mouse J774 macrophage cells: Cell surface detection of P2RY2 in live intact mouse J774 macrophage cells: Black line: Cells. Black line: Cells + rabbit IgG isotype control-FITC. Black line: Cells + Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F), (2.5 µg).

product information

CAT :

APR-102-F

SKU :

APR-102-F_50 mcl

Product Name :

Anti-P2Y2 Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41232

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR102

Homology :

Rat, mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, C-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2

Product Page - Scientific background :

P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation1.The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y142-4.The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway5. It has become a leading target for the treatment of cystic fibrosis. Activation of P2RY2 increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis6.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 2, P2RY2, P2U1

Short Description :

A Rabbit Polyclonal Antibody to P2Y2 Receptor Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize P2RY2 from rat, mouse, and human samples. Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

P2Y purinoceptor 2, P2RY2, P2U1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-102), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage