Anti-Plexin-C1 (extracellular)-FITC Antibody | Alomone Labs APR-091-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Plexin-C1 (extracellular)-FITC Antibody

catalog :

APR-091-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Plexin-C1 by direct flow cytometry in live intact mouse J774 macrophage cell line: Cell surface detection of Plexin-C1 by direct flow cytometry in live intact mouse J774 macrophage cell line: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F), (2.5µg).

product information

CAT :

APR-091-F

SKU :

APR-091-F_50 mcl

Product Name :

Anti-Plexin-C1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O60486

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR091

Homology :

Mouse - identical Rat - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1

Product Page - Scientific background :

Plexin-C1 also known as Virus-encoded semaphorin protein receptor, is a ∼210 kDa type I transmembrane receptor that regulates cytoskeletal remodeling and adhesion in human melanocytes.It belongs to the family of Plexin receptors, these are type I transmembrane proteins (A-D) that share a highly conserved cytoplasmic domain and an intracellular region containing an intrinsic Ras-GTPase activating (GAP) domain. Plexin receptors bind semaphorins: a class of secreted and membrane proteins 1. The natural ligand of Plexin C1 is Semaphorin -7A (#ASR-077).Plexin-C1 is highly expressed in melanocytes, neurons, neutrophils, dendritic cells and pre-osteoblasts and it has a complex effect on migration of melanocytes and melanoma, that depends on the phase of progression of this type of cancer 2,3. It also has additional roles in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, in the development of inflamation and in inhibition of neutrophils phagocytosis 3,4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1

Short Description :

A Rabbit Polyclonal Antibody to PLXNC1 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Plexin-C1 (extracellular) Antibody (#APR-091) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize PLXNC1 from mouse, rat and human samples. Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize PLXNC1 from mouse, rat and human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PLXNC1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-091), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage