Anti-Plexin-A4 (extracellular) Antibody | Alomone Labs APR-084 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Plexin-A4 (extracellular) Antibody

catalog :

APR-084

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of human THP-1 monocytic leukemia cell line lysate:

image 3 :

Expression of Plexin-A4 in rat hippocampus.

product information

CAT :

APR-084

SKU :

APR-084-CF_0.2 ml

Product Name :

Anti-Plexin-A4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80UG2

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR084

Homology :

Human, Rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RKLTKNSEADGMFAY, corresponding to amino acid residues 201 - 215 of mouse Plexin-A4

Product Page - Scientific background :

The plexin family are transmembrane proteins which are classified into four subfamilies: plexin -A, B, C and D. The plexins serve as a receptor for seamaphorins (Sema), axon guidance molecules involved in cellular processes such as axon pruning and repulsion, regulation of cell migration, vascular remodeling and more1. All family members consist of a Sema domain at the N-terminus responsible for Sema binding. In addition, plexins have PSI and IPT (Integrin, Plexin, and Transcription factor) domains, which connect to the transmembrane (TM) helix. Moreover, intracellular region of all plexins contains a membrane proximal juxtamembrane region (JM), a Rho GTPase-binding domain (RBD), and GTPase Activating Protein (GAP) domain2.Plexin-A4 acts as a receptor for the secreted semaphorins Sema3A and Sema6A in the central nervous system, and mediates axon repulsion and synaptic plasticity. Moreover, genome-wide significant association study (GWAS) on humans, has identified that several single nucleotide polymorphisms (SNPs) in PLXNA4 can increase the risk of Alzheimer's disease (AD) 3,4.In the immune system, Plexin-A4 has been implicated in macrophage Toll-like receptor (TLR)-mediated signaling in sepsis, negative regulation of T-cell mediated immune responses in cancer5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

PLXNA4

Short Description :

A Rabbit Polyclonal antibody to Plexin-A4 (extracellular)

Long Description :

Anti-Plexin-A4 (extracellular) Antibody (#APR-084) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Plexin-A4 from mouse, rat and human samples.

Negative Control :

BLP-PR084

Positive Control :

Synonyms :

PLXNA4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PLXNA4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage